Dolinnaia N G, Tsytovich A V, Sergeev V N, Gertsiuk M N, Shabarova Z A
Bioorg Khim. 1990 Sep;16(9):1183-94.
Chemical ligation of oligonucleotides in double stranded complexes has been considered in its structural-kinetic aspect. In a study of the reactivity of eleven synthetic water soluble carbodiimides the reaction rate has been found to be strongly affected by the substituents at N1 and N3 atoms. The chemical ligation appears to involve the linear form of carbodiimide. Model systems have been used to assess the rate constant for the phosphate activation. The ratio of kinetic constants for the productive and nonproductive reactions of the activated derivate apparently reflects the reactive site conformation; for a given duplex this parameter is virtually independent of the condensing agent structure.
双链复合物中寡核苷酸的化学连接已从其结构动力学方面进行了研究。在一项对十一种合成水溶性碳二亚胺反应活性的研究中，发现反应速率受N1和N3原子上取代基的强烈影响。化学连接似乎涉及碳二亚胺的线性形式。已使用模型系统来评估磷酸盐活化的速率常数。活化衍生物的生产性和非生产性反应的动力学常数之比显然反映了反应位点的构象；对于给定的双链体，该参数实际上与缩合剂结构无关。
