Dolinnaia N G, Tsytovich A V, Tevosian S G, Sergeev V N, Shabarova Z A
Bioorg Khim. 1990 Sep;16(9):1195-209.
To characterize structural factors affecting kinetic parameters of chemical ligation, DNA duplexes with various arrangements of reacting groups in the ligation site have been synthesized and studied. The modifications included replacement of a deoxythymidine with a uridine or a nucleoside having reversed configuration at C2' and/or C3' atoms, the modified residue being phosphate donor or acceptor; introduction of an "extra" nucleotide residue; replacement of a G.C pair second to the junction with a noncomplementary G.G pair. Thermal stability of the anomalous nucleotide duplexes has been characterized. Based on analysis of the kinetic parameters of chemical ligation, a suggestion is made about the conformation of the reactive site.
为了表征影响化学连接动力学参数的结构因素，已合成并研究了在连接位点具有各种反应基团排列的DNA双链体。这些修饰包括用尿苷或在C2'和/或C3'原子处具有反向构型的核苷取代脱氧胸苷，修饰的残基为磷酸供体或受体；引入一个“额外的”核苷酸残基；用非互补的G.G对取代连接点处的第二个G.C对。已表征了异常核苷酸双链体的热稳定性。基于对化学连接动力学参数的分析，对反应位点的构象提出了一种推测。
