Department of Physics of Biological Systems, Andronikashvili Institute of Physics, 6, Tamarashvili St, 0177 Tbilisi, Georgia.
Biol Trace Elem Res. 2011 Sep;142(3):388-97. doi: 10.1007/s12011-010-8806-y. Epub 2010 Sep 1.
The changes in glutathione-dependent cycle enzymes and catalase activities under Cr(VI)-induced oxidative stress were investigated in two distinct cell lines: L-41-human epithelial-like cells and HLF-fetal human diploid lung fibroblasts, which differ in tissue origin, proliferation, and antioxidant enzymes activities. The chromium concentrations from 1 to 5 μM cause nontoxic effects and activate antioxidant enzymes to overcome oxidative stress. In spite of some differences in the endogenous antioxidant activities, both cell lines reveal the same range of toxic concentrations (20-30 μM). The irreversible inhibition of glutathione-dependent antioxidant enzymes develops under toxic concentrations and serves as a marker of toxicity. The endogenous antioxidant activity influences time-dependent expression of Cr(VI) toxicity and the dynamics of antioxidant enzymes activity under nontoxic conditions. The cell antioxidant defense system is an important marker of the cell adaptive capacity under nontoxic and toxic conditions.
研究了两种不同细胞系（L-41 人上皮样细胞和 HLF 胎儿人二倍体肺成纤维细胞）中谷胱甘肽依赖循环酶和过氧化氢酶活性在 Cr(VI)诱导的氧化应激下的变化，这两种细胞系在组织来源、增殖和抗氧化酶活性方面存在差异。浓度为 1 至 5 μM 的铬会产生无毒作用，并激活抗氧化酶以克服氧化应激。尽管内源性抗氧化活性存在一些差异，但两种细胞系都显示出相同的毒性浓度范围（20-30 μM）。在毒性浓度下，谷胱甘肽依赖的抗氧化酶会发生不可逆抑制，从而成为毒性的标志物。内源性抗氧化活性会影响 Cr(VI)毒性的时程表达以及非毒性条件下抗氧化酶活性的动态变化。细胞抗氧化防御系统是在非毒性和毒性条件下细胞适应能力的重要标志物。
