Rizzi M, Cravello B, Renò F
Innovative Research Laboratory on Wound Healing, Health Sciences Department, University of Eastern Piedmont, Novara, 28100, Italy.
Cell Prolif. 2014 Dec;47(6):578-86. doi: 10.1111/cpr.12132. Epub 2014 Sep 16.
Textiles used to make clothing can represent a source, often ignored, of chemicals potentially noxious to both skin and the whole organism. Among the most frequently produced potentially noxious chemical manufacturing by-products are formaldehyde (FA), nickel (Ni) and hexavalent chromium (Cr); they are of potential clinical interest as all are known to be carcinogenic to humans and to be potent skin sensitizers. The aim of this study was to investigate, in vitro, effects of these potentially dangerous compounds on two different melanoma cell lines. In particular, attention was focused on A375P, a poorly metastatic and low invasive cell line and SK-MEL-28, a highly metastatic cell line.
Effects of these compounds was evaluated on A375P and SK-MEL-28 cells. FA (1-5 × 10(-5) m), NiSO4 (10(-6) -10(-3) m), K2 Cr2 O7 (10(-7) -10(-6) m) effects on cell proliferation were evaluated by cell counting, while ERK pathway involvement was evaluated by Western blot analysis.
Low concentrations of the chemicals, covering a range that corresponds to commonly accepted limits in textile production, induced a significant increase in cell proliferation concomitant with transient activation of phosphorylated ERK expression.
Data obtained suggest that increasing attention must be focused on these by-products' potentially harmful effects in chemical manufacturing of clothes and accessories, that remain for long periods of time, in contact with human skin.
用于制作服装的纺织品可能是对皮肤和整个机体都有潜在危害的化学物质的一个常被忽视的来源。在最常产生的潜在有害化学制造副产品中，有甲醛（FA）、镍（Ni）和六价铬（Cr）；它们都已知对人类具有致癌性且是强效皮肤致敏剂，因此具有潜在的临床意义。本研究的目的是在体外研究这些潜在危险化合物对两种不同黑色素瘤细胞系的影响。特别关注的是A375P，一种低转移性和低侵袭性的细胞系，以及SK - MEL - 28，一种高转移性细胞系。
评估这些化合物对A375P和SK - MEL - 28细胞的影响。通过细胞计数评估FA（1 - 5×10⁻⁵ m）、NiSO₄（10⁻⁶ - 10⁻³ m）、K₂Cr₂O₇（10⁻⁷ - 10⁻⁶ m）对细胞增殖的影响，同时通过蛋白质免疫印迹分析评估ERK途径的参与情况。
低浓度的这些化学物质，其范围对应于纺织品生产中普遍接受的限度，可诱导细胞增殖显著增加，并伴随磷酸化ERK表达的短暂激活。
所获得的数据表明，必须更加关注这些副产品在长期与人体皮肤接触的服装和配饰化学制造过程中潜在的有害影响。
