Collard Jean-Francois, Mertens Benjamin, Hinsenkamp Maurice
Université Libre de Bruxelles, Brussels, Belgium.
Bioelectromagnetics. 2011 Jan;32(1):28-36. doi: 10.1002/bem.20608.
An acceleration of differentiation, at the expense of proliferation, is observed after exposure of various biological models to low frequency and low amplitude electric and electromagnetic fields. Following these results showing significant modifications, we try to identify the biological mechanism involved at the cell level through microarray screening. For this study, we use epidermis cultures harvested from human abdominoplasty. Two platinum electrodes are used to apply the electric signal. The gene expressions of 38,500 well-characterized human genes are analyzed using Affymetrix(®) microarray U133 Plus 2.0 chips. The protocol is repeated on three different patients. After three periods of exposure, a total of 24 chips have been processed. After the application of ELF electric fields, the microarray analysis confirms a modification of the gene expression of epidermis cells. Particularly, four up-regulated genes (DKK1, TXNRD1, ATF3, and MME) and one down-regulated gene (MACF1) are involved in the regulation of proliferation and differentiation. Expression of these five genes was also confirmed by real-time rtPCR in all samples used for microarray analysis. These results corroborate an acceleration of cell differentiation at the expense of cell proliferation.
在将各种生物模型暴露于低频低振幅电场和电磁场后，观察到以增殖为代价的分化加速现象。基于这些显示出显著变化的结果，我们试图通过微阵列筛选来确定细胞水平上涉及的生物学机制。在本研究中，我们使用从人体腹部整形术中获取的表皮培养物。使用两个铂电极施加电信号。使用Affymetrix（®）微阵列U133 Plus 2.0芯片分析38500个特征明确的人类基因的基因表达。该方案在三名不同患者身上重复进行。经过三个暴露周期后，共处理了24个芯片。在施加极低频电场后，微阵列分析证实了表皮细胞基因表达的改变。特别是，四个上调基因（DKK1、TXNRD1、ATF3和MME）和一个下调基因（MACF1）参与了增殖和分化的调节。在用于微阵列分析的所有样本中，通过实时rtPCR也证实了这五个基因的表达。这些结果证实了以细胞增殖为代价的细胞分化加速。
