Department of Physics, Clarkson University, Potsdam, New York 13676, USA.
J Phys Chem B. 2010 Sep 23;114(37):12166-74. doi: 10.1021/jp105912e.
Experimental and theoretical analyses of the lactate dehydrogenase and glutathione reductase based enzymatic AND logic gates in which the enzymes and their substrates serve as logic inputs are performed. These two systems are examples of the novel, previously unexplored class of biochemical logic gates that illustrate potential biomedical applications of biochemical logic. They are characterized by input concentrations at logic 0 and 1 states corresponding to normal and pathophysiological conditions. Our analysis shows that the logic gates under investigation have similar noise characteristics. Both significantly amplify random noise present in inputs; however, we establish that for realistic widths of the input noise distributions, it is still possible to differentiate between the logic 0 and 1 states of the output. This indicates that reliable detection of pathophysiological conditions is indeed possible with such enzyme logic systems.
对基于乳酸脱氢酶和谷胱甘肽还原酶的酶促与门进行了实验和理论分析，其中酶及其底物用作逻辑输入。这两个系统是新型的、以前未探索过的生化逻辑门的示例，说明了生化逻辑在潜在的生物医学应用中的价值。它们的特点是逻辑 0 和 1 状态的输入浓度分别对应于正常和病理生理条件。我们的分析表明，所研究的逻辑门具有相似的噪声特性。两者都显著放大了输入中的随机噪声；然而，我们确定，对于输入噪声分布的实际宽度，仍然有可能区分输出的逻辑 0 和 1 状态。这表明，使用这种酶逻辑系统确实可以可靠地检测病理生理状况。
