Anal Chem. 2011 Nov 15;83(22):8383-6. doi: 10.1021/ac202139m. Epub 2011 Oct 17.
Biomolecular logic systems processing biochemical input signals and producing "digital" outputs in the form of YES/NO were developed for analysis of physiological conditions characteristic of liver injury, soft tissue injury, and abdominal trauma. Injury biomarkers were used as input signals for activating the logic systems. Their normal physiological concentrations were defined as logic-0 level, while their pathologically elevated concentrations were defined as logic-1 values. Since the input concentrations applied as logic 0 and 1 values were not sufficiently different, the output signals being at low and high values (0, 1 outputs) were separated with a short gap making their discrimination difficult. Coupled enzymatic reactions functioning as a biomolecular signal processing system with a built-in filter property were developed. The filter process involves a partial back-conversion of the optical-output-signal-yielding product, but only at its low concentrations, thus allowing the proper discrimination between 0 and 1 output values.
为了分析肝损伤、软组织损伤和腹部创伤等生理状况的特征，开发了生物分子逻辑系统，用于处理生化输入信号，并以“是/否”的形式产生“数字”输出。损伤生物标志物被用作激活逻辑系统的输入信号。它们的正常生理浓度被定义为逻辑 0 水平，而病理升高的浓度被定义为逻辑 1 值。由于用作逻辑 0 和 1 值的输入浓度没有足够的差异，因此处于低和高值（0、1 输出）的输出信号之间的间隔很短，使得它们难以区分。开发了一种耦合酶反应，作为具有内置滤波特性的生物分子信号处理系统。该过滤过程涉及光输出信号产物的部分反向转化，但仅在其低浓度下，从而允许在 0 和 1 输出值之间进行适当的区分。
