争议能否尘埃落定？

Can controversies be put to REST?

机构信息

Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.

出版信息

Nature. 2010 Sep 2;467(7311):E3-4; discussion E5. doi: 10.1038/nature09305.

DOI:10.1038/nature09305

PMID:20811409

Abstract

The contribution of REST to embryonic stem (ES) cell pluripotency has been uncertain. Two years ago, Singh et al. claimed that Rest(+/-) and REST knock-down ES cells expressed reduced levels of pluripotency markers, in contrast to a prior and subsequent reports. To understand the basis of this difference, we analysed the YHC334 (YHC) and RRC160 (RRC) gene-trap ES cell lines used by Singh et al., obtained directly from BayGenomics. Both REST mutant lines generated REST-betaGeo fusion proteins, but expressed pluripotency genes at levels similar to appropriately matched parental wild ES cells, consistent with expression being REST-independent.

摘要

REST 对胚胎干细胞（ES 细胞）多能性的贡献一直存在不确定性。两年前，Singh 等人声称，与之前和之后的报告相反，Rest(+/-)和 REST 敲低 ES 细胞表达的多能性标志物水平降低。为了理解这种差异的基础，我们分析了 Singh 等人直接从 BayGenomics 获得的 YHC334（YHC）和 RRC160（RRC）基因捕获 ES 细胞系。这两个 REST 突变系都产生了 REST-βGeo 融合蛋白，但表达多能性基因的水平与适当匹配的亲本野生型 ES 细胞相似，这与表达是 REST 非依赖性的一致。