Lymphocyte Development Group, MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, UK.
Nature. 2010 Sep 2;467(7311):E3-4; discussion E5. doi: 10.1038/nature09305.
The contribution of REST to embryonic stem (ES) cell pluripotency has been uncertain. Two years ago, Singh et al. claimed that Rest(+/-) and REST knock-down ES cells expressed reduced levels of pluripotency markers, in contrast to a prior and subsequent reports. To understand the basis of this difference, we analysed the YHC334 (YHC) and RRC160 (RRC) gene-trap ES cell lines used by Singh et al., obtained directly from BayGenomics. Both REST mutant lines generated REST-betaGeo fusion proteins, but expressed pluripotency genes at levels similar to appropriately matched parental wild ES cells, consistent with expression being REST-independent.
REST 对胚胎干细胞（ES 细胞）多能性的贡献一直存在不确定性。两年前，Singh 等人声称，与之前和之后的报告相反，Rest(+/-)和 REST 敲低 ES 细胞表达的多能性标志物水平降低。为了理解这种差异的基础，我们分析了 Singh 等人直接从 BayGenomics 获得的 YHC334（YHC）和 RRC160（RRC）基因捕获 ES 细胞系。这两个 REST 突变系都产生了 REST-βGeo 融合蛋白，但表达多能性基因的水平与适当匹配的亲本野生型 ES 细胞相似，这与表达是 REST 非依赖性的一致。
