Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Henan Key Laboratory of Tumor Pathology, Zhengzhou, Henan 450052, P.R. China.
Oncol Rep. 2010 Oct;24(4):997-1004.
It is well documented that Provirus integration site for Moloney murine leukemia virus 1 (Pim-1), as a proto-oncogene encoding a serine/threonine kinase with multiple cellular functions, is tightly associated with the occurrence and development of tumors. Overexpression of Pim-1 plays a critical role in the progression of several different tumors. In this study, esophageal squamous cell carcinoma (ESCC) EC9706 cells with Pim-1 siRNA treatment resulted in a clear decrease of Pim-1 levels, followed by inhibiting cell proliferation and inducing apoptosis. Further, Pim-1 siRNA reduced phosphorylation of Akt and Bad, and increased cleaved caspase-3/-9 activities and expression levels. These data suggest that Pim-1 siRNA-mediated apoptosis is closely related to the decrease in Akt and Bad phosphorylation and increase in cleaved caspase-3/-9 activities, and thus manipulation of Pim-1 is a potential target for molecular therapy in the clinical treatment of patients with ESCC.
有大量文献记载，莫洛尼鼠白血病病毒 1 的前病毒整合位点（Pim-1）作为一个编码具有多种细胞功能的丝氨酸/苏氨酸激酶的原癌基因，与肿瘤的发生和发展密切相关。Pim-1 的过表达在几种不同肿瘤的进展中起着关键作用。在这项研究中，用 Pim-1 siRNA 处理食管鳞状细胞癌（ESCC）EC9706 细胞后，Pim-1 水平明显下降，随后抑制细胞增殖并诱导细胞凋亡。此外，Pim-1 siRNA 降低了 Akt 和 Bad 的磷酸化水平，增加了 cleaved caspase-3/-9 的活性和表达水平。这些数据表明，Pim-1 siRNA 介导的细胞凋亡与 Akt 和 Bad 磷酸化的降低以及 cleaved caspase-3/-9 活性的增加密切相关，因此操纵 Pim-1 是临床治疗 ESCC 患者的分子治疗的一个潜在靶点。
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