Laboratory for Cell Biology, Department of Bioengineering of Zhengzhou University, Zhengzhou, PR China.
J Surg Oncol. 2010 Nov 1;102(6):683-8. doi: 10.1002/jso.21627.
It is well known that provirus integration site for Moloney murine leukemia virus 1 (Pim-1) plays an essential role in the occurrence and development of tumors. Here, we investigated expression of Pim-1 in esophageal squamous cell carcinoma (ESCC) and assessed the correlation between Pim-1 level and prognosis of the patients with ESCC.
Expression of Pim-1 was investigated by RT-PCR, immunohistochemistry, and in situ hybridization methods. Kaplan-Meier statistics was used to examine the relationship between levels of Pim-1 and prognosis using log-rank test.
The results demonstrated that ESCC tissues appeared strong expression levels of Pim-1 mRNA and protein while the normal esophageal tissues were either negative or showed only weak Pim-1 mRNA and protein levels. In addition, we found that Pim-1 expression was significantly correlated with histology grade, clinical staging, and lymph node metastasis (all P < 0.05), but not related to age and sex (all P > 0.05). Furthermore, the results of survival rates analyzed by Kaplan-Meier curve revealed that patients with high Pim-1 mRNA and protein expressions had a poorer prognosis than those with the low Pim-1 expression (P = 0.010 and 0.001, respectively).
These findings demonstrate that Pim-1 may be used as molecular marker for predicting the prognosis of patients with ESCC.
众所周知，莫洛尼鼠白血病病毒 1（Pim-1）的前病毒整合位点在肿瘤的发生和发展中起着至关重要的作用。在这里，我们研究了 Pim-1 在食管鳞状细胞癌（ESCC）中的表达，并评估了 Pim-1 水平与 ESCC 患者预后之间的相关性。
通过 RT-PCR、免疫组织化学和原位杂交方法检测 Pim-1 的表达。采用 Kaplan-Meier 统计方法，通过对数秩检验检查 Pim-1 水平与预后之间的关系。
结果表明，ESCC 组织中 Pim-1 mRNA 和蛋白表达水平较强，而正常食管组织则为阴性或仅显示较弱的 Pim-1 mRNA 和蛋白水平。此外，我们发现 Pim-1 表达与组织学分级、临床分期和淋巴结转移显著相关（均 P < 0.05），但与年龄和性别无关（均 P > 0.05）。此外，Kaplan-Meier 曲线分析的生存率结果表明，Pim-1 mRNA 和蛋白高表达的患者预后较 Pim-1 低表达的患者差（P = 0.010 和 0.001）。
这些发现表明，Pim-1 可作为预测 ESCC 患者预后的分子标志物。
