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诱导型微小RNA-15b的抑制可防止心脏功能不全的Dicer缺失模型中心脏功能的快速丧失。

Suppression of Induced microRNA-15b Prevents Rapid Loss of Cardiac Function in a Dicer Depleted Model of Cardiac Dysfunction.

作者信息

Roy Sashwati, Banerjee Jaideep, Gnyawali Surya C, Khanna Savita, He Guanglong, Pfeiffer Douglas, Zweier Jay L, Sen Chandan K

机构信息

Davis Heart and Lung Research Institute and Department of Surgery, The Ohio State University Medical Center, Columbus, Ohio, United States of America.

出版信息

PLoS One. 2013 Jun 19;8(6):e66789. doi: 10.1371/journal.pone.0066789. Print 2013.

DOI:10.1371/journal.pone.0066789
PMID:23840532
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3686742/
Abstract

BACKGROUND

Dicer endonuclease, critical for maturation of miRNAs, is depleted in certain forms of cardiomyopathy which results in differential expression of certain microRNAs. We sought to elucidate the mechanisms underlying the rapid loss of cardiac function following cardiac-specific Dicer depletion in adult mice.

RESULTS

Conditional Dicer deletion in the adult murine myocardium demonstrated compromised heart function, mitochondrial dysfunction and oxidant stress. Elevated miR-15b was observed as an early response to Dicer depletion and was found to silence Pim-1 kinase, a protein responsible for maintaining mitochondrial integrity and function. Anti-miRNA based suppression of induced miRNA-15b rescued the function of Dicer-depleted adult heart and attenuated hypertrophy.

CONCLUSIONS

Anti-miRNA based suppression of inducible miRNA-15b can prevent rapid loss of cardiac function in a Dicer-depleted adult heart and can be a key approach worthy of therapeutic consideration.

摘要

背景

Dicer核酸内切酶对微小RNA（miRNA）的成熟至关重要，在某些形式的心肌病中会减少，这导致某些微小RNA的差异表达。我们试图阐明成年小鼠心脏特异性Dicer缺失后心脏功能迅速丧失的潜在机制。

结果

成年小鼠心肌中条件性Dicer缺失表现为心脏功能受损、线粒体功能障碍和氧化应激。观察到miR-15b升高是对Dicer缺失的早期反应，并且发现其使Pim-1激酶沉默，Pim-1激酶是一种负责维持线粒体完整性和功能的蛋白质。基于抗miRNA对诱导型miRNA-15b的抑制挽救了Dicer缺失的成年心脏的功能并减轻了肥大。

结论

基于抗miRNA对诱导型miRNA-15b的抑制可预防Dicer缺失的成年心脏中心脏功能的快速丧失，并且可能是值得治疗考虑的关键方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/fb41cd54ac21/pone.0066789.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/5ba83fa3eedb/pone.0066789.g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/1689d846c421/pone.0066789.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/3f169240e412/pone.0066789.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/339bf9b3a310/pone.0066789.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/3148c2987104/pone.0066789.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/fb41cd54ac21/pone.0066789.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/5ba83fa3eedb/pone.0066789.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/375bc9161f20/pone.0066789.g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/339bf9b3a310/pone.0066789.g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9196/3686742/fb41cd54ac21/pone.0066789.g008.jpg

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PLoS One. 2012;7(2):e30933. doi: 10.1371/journal.pone.0030933. Epub 2012 Feb 3.
2
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Circ Res. 2012 Jan 6;110(1):71-81. doi: 10.1161/CIRCRESAHA.111.244442. Epub 2011 Nov 3.
3
Mechanism of transfer of functional microRNAs between mouse dendritic cells via exosomes.小鼠树突状细胞间功能性 microRNAs 通过外泌体转移的机制。
机器学习和生物信息学鉴定扩张型心肌病中 8 个自噬相关生物标志物并构建基因调控网络。
Sci Rep. 2022 Sep 2;12(1):15030. doi: 10.1038/s41598-022-19027-5.
4
Preliminary construction of a regulatory network of miRNAs in the pathogenesis of nucleus pulposus degeneration - a review based on data mining.基于数据挖掘的髓核退变发病机制中miRNA调控网络的初步构建——综述
Am J Transl Res. 2021 Sep 15;13(9):9919-9931. eCollection 2021.
5
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Cancers (Basel). 2021 Apr 29;13(9):2139. doi: 10.3390/cancers13092139.
6
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Int J Nanomedicine. 2020 Mar 31;15:2287-2302. doi: 10.2147/IJN.S240301. eCollection 2020.
7
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8
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J Vis Exp. 2010 Jul 9(41):1781. doi: 10.3791/1781.