血涂片中二倍体、三倍体和四倍体鱼类红细胞的图像细胞测量反映了活细胞的真实尺寸。

Image cytometric measurements of diploid, triploid and tetraploid fish erythrocytes in blood smears reflect the true dimensions of live cells.

机构信息

University of South Bohemia esk Budjovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, 389 25 Vodany, Czech Republic.

出版信息

Cell Biol Int. 2011 Jan;35(1):67-71. doi: 10.1042/CBI20100198.

DOI:10.1042/CBI20100198

PMID:20812918

Abstract

Comparative image cytometry of erythrocytes of diploid and triploid tench Tinca tinca L. and evolutionary tetraploid sterlet Acipenser ruthenus L. was performed on whole live unstained cells, live cells with stained nuclei and on stained fixed whole cells and their nuclei to test if erythrocyte measurements made from blood smears reflect the true dimensions of live cells. Nuclear area and perimeter were the best ploidy level predictors distinguishing accurately among live and fixed diploid, triploid and tetraploid cells, without significant differences between live and fixed cells within a ploidy level. Redundancy analysis revealed insignificant marginal effect of fixation (explained 2.3% of variation, F = 0.804), whereas the effect of ploidy level was highly significant (explained 50.6% of variation, F = 34.874). The erythrocyte measurements of diploid, triploid and tetraploid fish erythrocytes and their nuclei made from blood smears reflect the true dimensions of live cells, and the fixation procedure did not substantially affect their predictive value for ploidy level determination.

摘要

对二倍体和三倍体欧鳊（Tinca tinca L.）以及进化的四倍体欧洲鳇（Acipenser ruthenus L.）的红细胞进行了整体活未染色细胞、活细胞核染色细胞以及固定完整细胞和核的比较图像细胞计量学分析，以测试从血涂片测量的红细胞是否反映了活细胞的真实尺寸。核面积和周长是区分活细胞和固定细胞的最佳倍性水平预测指标，可以准确地区分二倍体、三倍体和四倍体细胞，而在同一倍性水平内，活细胞和固定细胞之间没有显著差异。冗余分析显示固定的边缘效应不显著（解释了 2.3%的变异，F = 0.804），而倍性水平的效应高度显著（解释了 50.6%的变异，F = 34.874）。从血涂片测量的二倍体、三倍体和四倍体鱼的红细胞及其核的红细胞测量值反映了活细胞的真实尺寸，并且固定过程并没有显著影响其确定倍性水平的预测值。