Department of Orthopaedics of Taizhou Hospital, Linhai 317000, Zhejiang, China.
Neurosci Lett. 2010 Nov 12;485(1):60-4. doi: 10.1016/j.neulet.2010.08.063. Epub 2010 Sep 9.
Oxidative stress is one of the several mechanisms that induces apoptosis in cells. It has been shown that hydrogen peroxide (H(2)O(2)) induces apoptosis in several kinds of cells; however, the role of H(2)O(2) in the apoptosis of Schwann cells (SCs) is currently unclear. The objective of this study was to determine whether H(2)O(2) is capable of inducing apoptosis in SCs and whether or not such an effect is associated with the activation of mitochondrial pathway. We demonstrated that H(2)O(2) induces apoptosis in SCs, and is associated with increased release of cytochrome c from mitochondria and the activation of caspase-3 and -9 by up-regulation of Bax and down-regulation of Bcl-2. These results suggest a potential role for H(2)O(2) in SC injury by triggering apoptosis via the mitochondrial pathway under oxidative stress.
氧化应激是诱导细胞凋亡的几种机制之一。已经表明，过氧化氢 (H2O2) 可诱导多种细胞凋亡；然而，H2O2 在许旺细胞 (SCs) 凋亡中的作用目前尚不清楚。本研究旨在确定 H2O2 是否能够诱导SCs 凋亡，以及这种效应是否与线粒体途径的激活有关。我们证明 H2O2 可诱导SCs 凋亡，并与线粒体细胞色素 c 的释放增加以及 Bax 的上调和 Bcl-2 的下调导致 caspase-3 和 -9 的激活有关。这些结果表明，在氧化应激下，H2O2 通过触发线粒体途径的凋亡，在 SC 损伤中可能发挥作用。
