Rani M, Gupta R K, Chhibber S
Department of Microbiology, Panjab University, Chandigarh, India.
Can J Microbiol. 1990 Dec;36(12):885-90. doi: 10.1139/m90-153.
The immunoprotective role of lipopolysaccharide and related antigens from Klebsiella pneumoniae was studied in a lobar pneumonia model developed in rats. Various antigens were obtained by different chemical treatments of the lipopolysaccharide. All these antigens (purified lipopolysaccharide, reduced lipopolysaccharide, lipopolysaccharide--bovine serum albumin complex, and lipid A--bovine serum albumin complex were tested for pyrogenicity and the Shwartzman reaction. The lipopolysaccharide and the various related antigens were pyrogenic and elicited a positive Shwartzman reaction at high concentrations. However, at low concentrations, the same preparations did not show any side effects. All these antigens, on the other hand, were protective against bacterial challenge in Klebsiella pneumoniae induced lobar pneumonia in rats, as the bacterial colonization of lungs in the immunized animals was significantly lower when compared with the controls. The alveolar macrophages from these animals also showed significantly more uptake of Klebsiella pneumoniae as compared with those obtained from control animals.
在大鼠建立的大叶性肺炎模型中，研究了肺炎克雷伯菌脂多糖及相关抗原的免疫保护作用。通过对脂多糖进行不同的化学处理获得了各种抗原。对所有这些抗原（纯化的脂多糖、还原脂多糖、脂多糖 - 牛血清白蛋白复合物和脂质A - 牛血清白蛋白复合物）进行了热原性和施瓦茨曼反应测试。脂多糖和各种相关抗原具有热原性，在高浓度时可引发阳性施瓦茨曼反应。然而，在低浓度时，相同的制剂未显示任何副作用。另一方面，所有这些抗原在大鼠肺炎克雷伯菌诱导的大叶性肺炎中对细菌攻击具有保护作用，因为与对照组相比，免疫动物肺部的细菌定植明显更低。与对照组动物相比，这些动物的肺泡巨噬细胞对肺炎克雷伯菌的摄取也明显更多。
