Chhibber S, Bajaj J
Department of Microbiology, Panjab University, Chandigarh, India.
Vaccine. 1995 Feb;13(2):179-84. doi: 10.1016/0264-410x(95)93133-t.
Klebsiella pneumoniae has become an important cause of both community-acquired and nosocomial infections. In this study an attempt was made to study the immunogenicity of iron-regulated cell surface proteins (IRCSP) alone or in conjunction with the polysaccharide moiety of lipopolysaccharide (LPS) of K. pneumoniae. The polysaccharide-iron-regulated cell surface protein conjugate (PS-IRCSP) was non-toxic and non-pyrogenic. It was found to be immunogenic and the protection afforded by the conjugate against the challenge strain was observed in a rat lobar pneumonia model. The protection observed with the conjugate was higher than that observed with polysaccharide or IRCSP alone. The conjugate elicited both agglutinating and bactericidal antibodies. Enhanced phagocytosis was observed for the alveolar macrophages obtained from the lungs of animals treated with conjugate compared with macrophages obtained from animals treated with other antigenic preparations.
肺炎克雷伯菌已成为社区获得性感染和医院感染的重要病因。在本研究中，我们尝试单独研究铁调节细胞表面蛋白（IRCSP）或其与肺炎克雷伯菌脂多糖（LPS）多糖部分联合使用时的免疫原性。多糖-铁调节细胞表面蛋白偶联物（PS-IRCSP）无毒且无致热原性。研究发现其具有免疫原性，并且在大鼠大叶性肺炎模型中观察到该偶联物对攻击菌株的保护作用。观察到的偶联物的保护作用高于单独使用多糖或IRCSP时的保护作用。该偶联物可诱导凝集抗体和杀菌抗体。与用其他抗原制剂处理的动物的巨噬细胞相比，在用偶联物处理的动物的肺中获得的肺泡巨噬细胞观察到吞噬作用增强。
