Kwong L, Xie Y J, Daniel J, Robbins S M, Weeks G
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Development. 1990 Sep;110(1):303-10. doi: 10.1242/dev.110.1.303.
The stalk cell differentiation inducing factor (DIF) has the properties required of a morphogen responsible for pattern regulation during the pseudoplasmodial stage of Dictyostelium development. It induces prestalk cell formation and inhibits prespore cell formation, but there is as yet no strong evidence for a morphogenetic gradient of DIF. We have measured DIF accumulation by monolayers of isolated prestalk and prespore cells in an attempt to provide evidence for such a gradient. DIF is accumulated in the largest quantities by a subpopulation of prestalk cells that specifically express the DIF-inducible genes pDd56 and pDd26. Since it has been shown recently that cells that express pDd56 are localized in the central core of the prestalk cell region of the pseudoplasmodia, our current results suggest a morphogenetic gradient generated by this region.
柄细胞分化诱导因子（DIF）具有在盘基网柄菌发育的伪原质团阶段负责模式调控的形态发生素所需的特性。它诱导柄细胞前体形成并抑制孢子前体细胞形成，但目前尚无确凿证据表明存在DIF的形态发生梯度。我们通过分离的柄细胞前体和孢子前体细胞单层来测量DIF的积累，试图为这种梯度提供证据。DIF在特异性表达DIF诱导基因pDd56和pDd26的柄细胞前体亚群中积累量最大。由于最近已表明表达pDd56的细胞位于伪原质团柄细胞前体区域的中央核心，我们目前的结果表明该区域产生了一种形态发生梯度。
