Department of Infectious Diseases, Virology, University of Heidelberg, Heidelberg, Germany.
Eur J Clin Microbiol Infect Dis. 2011 Jan;30(1):121-5. doi: 10.1007/s10096-010-1046-4. Epub 2010 Sep 3.
Resistance to linezolid has been associated with a G2576T mutation in the 23 S rRNA gene. Clinical isolates of linezolid-sensitive and linezolid-resistant vancomycin-resistant Enterococcus faecium of a liver transplant patient have been analysed for the G2576T mutation by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), conventional sequencing and pyrosequencing. A clear association between the number of mutated 23 S rRNA genes and the level of linezolid resistance has been demonstrated. Linezolid susceptibility re-emerged after cessation of linezolid therapy; however, the re-initiation of linezolid therapy resulted in the re-emergence of linezolid-resistant strains. Pyrosequencing rapidly detected the number of mutated alleles and is superior to conventional PCR-RFLP for the detection of heterozygous mutations.
对利奈唑胺的耐药性与 23S rRNA 基因中的 G2576T 突变有关。通过聚合酶链反应限制性片段长度多态性（PCR-RFLP）、常规测序和焦磷酸测序分析了肝移植患者的利奈唑胺敏感和利奈唑胺耐药万古霉素耐药粪肠球菌的临床分离株的 G2576T 突变。已证明突变的 23S rRNA 基因数量与利奈唑胺耐药水平之间存在明确关联。停止利奈唑胺治疗后，利奈唑胺的敏感性重新出现；然而，重新开始利奈唑胺治疗导致了利奈唑胺耐药株的重新出现。焦磷酸测序可快速检测突变等位基因的数量，并且比常规 PCR-RFLP 更适合检测杂合突变。
