利用废菜籽油和丙醇作为 3-羟基戊酸的前体，通过希瓦氏菌属生产聚（3-羟基丁酸-co-3-羟基戊酸酯）。
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Cupriavidus necator from waste rapeseed oil using propanol as a precursor of 3-hydroxyvalerate.
机构信息
Faculty of Chemistry, Department of Food Chemistry and Biotechnology, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic.
出版信息
Biotechnol Lett. 2010 Dec;32(12):1925-32. doi: 10.1007/s10529-010-0376-8. Epub 2010 Aug 12.
Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l(-1), respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l(-1) h(-1), respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.
废弃的菜籽油是一种有用的底物，可用于生产聚羟基烷酸酯（PHA），采用的是铜绿假单胞菌 H16。在分批补料模式下，我们分别获得了 138 g/L 和 105 g/L 的生物量和 PHA 产率。产率系数和比生产速率分别为 0.83 g PHA/g 油和 1.46 g/L·h。体积分数为 1%的丙醇显著促进了 PHA 和生物量的形成，并且将 3-羟基戊酸单元掺入到 PHA 结构中。因此，丙醇可用作生产聚（3-羟基丁酸-co-3-羟基戊酸）共聚物的 3-羟基戊酸的有效前体。在分批补料培养过程中，将丙醇浓度维持在 1%，从而使共聚物中 3-羟基戊酸的含量达到 8%。
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