Department of Environmental Science, Tarbiat Modares University, Noor, Iran.
Biotechnol Appl Biochem. 2011 May;58(3):203-11. doi: 10.1002/bab.29. Epub 2011 May 31.
The objective of the present study was to investigate the ability of Cupriavidus necator to produce poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) on various carbon sources in batch cultivation. These results show that C. necator produces poly-3-hydroxybutyrate from single carbon sources. The highest poly-3-hydroxybutyrate (P3HB) content was achieved at growth on fructose in the exponential growth phase. The maximum yield of the P3HV content was obtained when fructose was mixed with acetate. The highest content P3HB-co-3HV was also achieved by C. necator when we supplied C-excess and N- and P-normal conditions. These results indicate that C. necator accumulates high polyhydroxyalkanoates (PHA) content by depleting these elements in the culture medium. Nitrogen and phosphorus limitation has no significant effect on the PHA production, whereas C-excess leads to an increase in PHA formation of up to 92% PHAs of cell dry weight after growth on 5 g/L acetate and 40 g/L fructose.
本研究旨在探讨脱氮假单胞菌在分批培养中利用各种碳源生产聚（3-羟基丁酸-co-3-羟基戊酸）的能力。这些结果表明，脱氮假单胞菌可以从单一碳源生产聚 3-羟基丁酸。在果糖的指数增长阶段，聚 3-羟基丁酸（P3HB）的含量最高。当果糖与乙酸混合时，可获得最高的 P3HV 含量。当提供 C 过量和 N 和 P 正常条件时，脱氮假单胞菌也可获得最高的 P3HB-co-3HV 含量。这些结果表明，脱氮假单胞菌通过耗尽培养基中的这些元素来积累高含量的聚羟基烷酸（PHA）。氮和磷的限制对 PHA 的生产没有显著影响，而 C 过量会导致在 5 g/L 乙酸和 40 g/L 果糖上生长后，PHA 形成增加高达 92%的细胞干重 PHAs。
