Bramanti V, Tomassoni D, Bronzi D, Grasso S, Currò M, Avitabile M, Li Volsi G, Renis M, Ientile R, Amenta F, Avola R
Dept of Chemical Sciences, Section of Biochemistry and Molecular biology, University of Catania, Viale A Doria 6, 95125 Catania, Italy.
Neurochem Res. 2010 Dec;35(12):2070-7. doi: 10.1007/s11064-010-0256-6. Epub 2010 Sep 3.
In the present study, we evaluated the expression of some proliferation and differentiation markers in 15 DIV astrocyte cultures pretreated or not with 0.5 mM glutamate for 24 h and than maintained under chronic or acute treatment with 50 μM R(+)enantiomer or raceme alpha-lipoic acid (ALA). GFAP expression significantly increased after (R+)enantiomer acute-treatment and also in glutamate-pretreated ones. Vimentin expression increased after R(+)enantiomer acute-treatment, but it decreased after raceme acute-treatment. Nestin expression drastically increased after acute raceme-treatment in glutamate-pretreated or not cultures, but significantly decreased after (R+)enantiomer acute and chronic-treatments. Cyclin D1 expression increased in raceme acute-treated cultures pretreated with glutamate. MAP-kinase expression slightly increased after (R+)enantiomer acute treatment in glutamate-pretreated or unpretreated ones. These preliminary findings may better clarify antioxidant and metabolic role played by ALA in proliferating and differentiating astrocyte cultures suggesting an interactive cross-talk between glial and neuronal cells, after brain lesions or damages.
在本研究中，我们评估了在15天体外培养的星形胶质细胞中一些增殖和分化标志物的表达情况，这些细胞在预处理或未预处理的情况下用0.5 mM谷氨酸处理24小时，然后用50 μM R(+)对映体或外消旋α-硫辛酸（ALA）进行慢性或急性处理。R(+)对映体急性处理后以及在谷氨酸预处理的细胞中，胶质纤维酸性蛋白（GFAP）表达显著增加。波形蛋白表达在R(+)对映体急性处理后增加，但在外消旋体急性处理后降低。巢蛋白表达在谷氨酸预处理或未预处理的培养物中外消旋体急性处理后急剧增加，但在R(+)对映体急性和慢性处理后显著降低。细胞周期蛋白D1表达在谷氨酸预处理的外消旋体急性处理培养物中增加。在谷氨酸预处理或未预处理的细胞中，R(+)对映体急性处理后丝裂原活化蛋白激酶（MAP-激酶）表达略有增加。这些初步发现可能会更好地阐明ALA在增殖和分化的星形胶质细胞培养物中的抗氧化和代谢作用，提示在脑损伤或损害后神经胶质细胞与神经元细胞之间存在交互对话。
