Morton 兵豆提取物通过抑制细胞内活性氧水平在体外减轻血管紧张素 II 诱导的心肌细胞肥大。
Morton lentil extract attenuated angiotensin II-induced cardiomyocyte hypertrophy via inhibition of intracellular reactive oxygen species levels in vitro.
机构信息
Department of Food and Cereal Sciences, North Dakota State University, 1320 Albrecht Boulevard, Fargo, North Dakota 58105, USA.
出版信息
J Agric Food Chem. 2010 Oct 13;58(19):10382-8. doi: 10.1021/jf101648m.
The objective was to investigate whether a lentil (Morton) extract had any protective effect on cardiac hypertrophy, which is one of the most significant sequelae of cardiovascular diseases. High phenolic compounds (43.4 mg of GAE/g), including thirteen phenolic acid and two flavonoids, were detected in the acetone/water/acetic acid lentil extract. The extract showed strong antioxidant ability (105 μmol of TE/g). The effect of lentil extract on angiotensin (Ang) II-induced cardiac hypertrophy was examined. Results showed that pretreatment with lentil extract (25, 50, 100 μg/mL) significantly attenuated Ang II (0.1 μM)-induced hypertrophy by 18, 28, and 36% in rat cardiomycytes, respectively; lentil extract (12.5, 25, 50 μg/mL) attenuated Ang II (0.1 μM)-induced hypertrophy by 9, 17, and 25% in human cardiomycytes, respectively. Intracellular reactive oxygen species (ROS) levels were enhanced by Ang II treatment, and this stimulatory action was significantly attenuated (33% inhibition) by lentil extract (100 μg/mL) in rat cardiomyocytes and attenuated by 22% by 50 μg/mL lentil extract in human cardiomyocytes. In conclusion, Morton lentil extracts attenuated Ang II-induced rat and human cardiomyocytes hypertrophy via decreasing intracellular ROS levels.
目的在于探究兵豆（Morton）提取物对心血管疾病的重要后遗症之一——心肌肥大是否具有保护作用。从丙酮/水/乙酸兵豆提取物中检测到高含量的酚类化合物（43.4mgGAE/g），其中包括 13 种酚酸和 2 种类黄酮。提取物具有很强的抗氧化能力（105μmolTE/g）。兵豆提取物对血管紧张素（Ang）II 诱导的心肌肥大的影响进行了研究。结果表明，兵豆提取物（25、50、100μg/mL）预处理可分别显著减轻 Ang II（0.1μM）诱导的大鼠心肌细胞肥大 18%、28%和 36%；兵豆提取物（12.5、25、50μg/mL）可分别减轻 Ang II（0.1μM）诱导的人心肌细胞肥大 9%、17%和 25%。Ang II 处理会增强细胞内活性氧（ROS）水平，而兵豆提取物（100μg/mL）可显著减弱这种刺激作用（抑制 33%），50μg/mL 兵豆提取物可减弱人心肌细胞中的 ROS 水平，抑制率为 22%。综上所述，Morton 兵豆提取物通过降低细胞内 ROS 水平来减轻 Ang II 诱导的大鼠和人心肌细胞肥大。
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