Department of Food and Cereal Sciences, North Dakota State University, 1320 Albrecht Boulevard, Fargo, North Dakota 58105, USA.
J Agric Food Chem. 2010 Oct 13;58(19):10382-8. doi: 10.1021/jf101648m.
The objective was to investigate whether a lentil (Morton) extract had any protective effect on cardiac hypertrophy, which is one of the most significant sequelae of cardiovascular diseases. High phenolic compounds (43.4 mg of GAE/g), including thirteen phenolic acid and two flavonoids, were detected in the acetone/water/acetic acid lentil extract. The extract showed strong antioxidant ability (105 μmol of TE/g). The effect of lentil extract on angiotensin (Ang) II-induced cardiac hypertrophy was examined. Results showed that pretreatment with lentil extract (25, 50, 100 μg/mL) significantly attenuated Ang II (0.1 μM)-induced hypertrophy by 18, 28, and 36% in rat cardiomycytes, respectively; lentil extract (12.5, 25, 50 μg/mL) attenuated Ang II (0.1 μM)-induced hypertrophy by 9, 17, and 25% in human cardiomycytes, respectively. Intracellular reactive oxygen species (ROS) levels were enhanced by Ang II treatment, and this stimulatory action was significantly attenuated (33% inhibition) by lentil extract (100 μg/mL) in rat cardiomyocytes and attenuated by 22% by 50 μg/mL lentil extract in human cardiomyocytes. In conclusion, Morton lentil extracts attenuated Ang II-induced rat and human cardiomyocytes hypertrophy via decreasing intracellular ROS levels.
目的在于探究兵豆（Morton）提取物对心血管疾病的重要后遗症之一——心肌肥大是否具有保护作用。从丙酮/水/乙酸兵豆提取物中检测到高含量的酚类化合物（43.4mgGAE/g），其中包括 13 种酚酸和 2 种类黄酮。提取物具有很强的抗氧化能力（105μmolTE/g）。兵豆提取物对血管紧张素（Ang）II 诱导的心肌肥大的影响进行了研究。结果表明，兵豆提取物（25、50、100μg/mL）预处理可分别显著减轻 Ang II（0.1μM）诱导的大鼠心肌细胞肥大 18%、28%和 36%；兵豆提取物（12.5、25、50μg/mL）可分别减轻 Ang II（0.1μM）诱导的人心肌细胞肥大 9%、17%和 25%。Ang II 处理会增强细胞内活性氧（ROS）水平，而兵豆提取物（100μg/mL）可显著减弱这种刺激作用（抑制 33%），50μg/mL 兵豆提取物可减弱人心肌细胞中的 ROS 水平，抑制率为 22%。综上所述，Morton 兵豆提取物通过降低细胞内 ROS 水平来减轻 Ang II 诱导的大鼠和人心肌细胞肥大。
