Department of Food Science and Technology, Keimyung University, Daegu 42601, Korea.
Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Korea.
Molecules. 2021 Dec 23;27(1):59. doi: 10.3390/molecules27010059.
Excessive oxidative stress plays a role in hepatotoxicity and the pathogenesis of hepatic diseases. In our previous study, the phenolic extract of beluga lentil (BLE) showed the most potent in vitro antioxidant activity among extracts of four common varieties of lentils; thus, we hypothesized that BLE might protect liver cells against oxidative stress-induced cytotoxicity. BLE was evaluated for its protective effects against oxidative stress-induced hepatotoxicity in AML12 mouse hepatocytes and BALB/c mice. HO treatment caused a marked decrease in cell viability; however, pretreatment with BLE (25-100 μg/mL) for 24 h significantly preserved the viability of HO-treated cells up to about 50% at 100 μg/mL. As expected, BLE dramatically reduced intracellular reactive oxygen species (ROS) levels in a dose-dependent manner in HO-treated cells. Further mechanistic studies demonstrated that BLE reduced cellular ROS levels, partly by increasing expression of antioxidant genes. Furthermore, pretreatment with BLE (400 mg/kg) for 2 weeks significantly reduced serum levels of alanine transaminase and triglyceride by about 49% and 40%, respectively, and increased the expression and activity of glutathione peroxidase in CCl-treated BALB/c mice. These results suggest that BLE protects liver cells against oxidative stress, partly by inducing cellular antioxidant system; thus, it represents a potential source of nutraceuticals with hepatoprotective effects.
过量的氧化应激在肝毒性和肝脏疾病的发病机制中起作用。在我们之前的研究中，白藜芦醇扁豆（BLE）的酚类提取物在四种常见扁豆提取物中显示出最强的体外抗氧化活性；因此，我们假设 BLE 可能保护肝细胞免受氧化应激诱导的细胞毒性。BLE 被评估对 AML12 小鼠肝细胞和 BALB/c 小鼠的氧化应激诱导的肝毒性的保护作用。HO 处理导致细胞活力明显下降；然而，用 BLE（25-100μg/mL）预处理 24 小时可显著保持 HO 处理细胞的活力，在 100μg/mL 时约为 50%。如预期的那样，BLE 以剂量依赖性方式显著降低 HO 处理细胞中的细胞内活性氧（ROS）水平。进一步的机制研究表明，BLE 通过增加抗氧化基因的表达，部分降低细胞内 ROS 水平。此外，用 BLE（400mg/kg）预处理 2 周可使 CCl 处理的 BALB/c 小鼠的血清丙氨酸转氨酶和甘油三酯水平分别降低约 49%和 40%，并增加谷胱甘肽过氧化物酶的表达和活性。这些结果表明，BLE 通过诱导细胞抗氧化系统来保护肝细胞免受氧化应激，因此它是具有保肝作用的营养保健品的潜在来源。
