Winkler Carla, Hüper Katja, Wedekind Ann-Carina, Rochlitzer Sabine, Hartwig Christina, Müller Meike, Braun Armin, Krug Norbert, Hohlfeld Jens M, Erpenbeck Veit J
Fraunhofer Institute of Toxicology and Experimental Medicine, Hannover, Germany.
Exp Lung Res. 2010 Nov;36(9):522-30. doi: 10.3109/01902141003790148.
Pollen starch granules (PSGs) are allergen particles that get into contact with pulmonary surfactant and phagocytes in the terminal airways. In this study, the effects of surfactant protein D (SP-D) on the interaction of PSGs with phagocytes and on the pulmonary clearance of PSGs were determined. Fluorescently labeled PSGs were incubated in vitro with murine lung macrophages or dendritic cells (DCs) ± recombinant rat SP-D (rrSP-D). In addition, the effect of SP-D on uptake of PSGs by lung macrophages and DCs was studied in vivo. Furthermore, PSGs were instilled in Balb/c mice and the effects of SP-D on total lung clearance were assessed by optical imaging. SP-D treatment increased the number of PSG-positive macrophages and DCs in vitro. Furthermore, SP-D accelerated uptake/binding by alveolar macrophages and reduced the number of PSG-positive tissue macrophages and DCs at 24 hours. However, SP-D did not affect total lung clearance of PSGs and it did not enhance the T-cell proliferation induced by PSG-positive DCs. In conclusion, SP-D increased PSG-positive cells in vitro and accelerated PSG binding/uptake in vivo. The observed effects were limited to cellular clearance mechanisms and did not affect the total clearance of PSGs from the lung.
花粉淀粉颗粒（PSGs）是一种变应原颗粒，可与终末气道中的肺表面活性物质和吞噬细胞接触。在本研究中，测定了表面活性蛋白D（SP-D）对PSGs与吞噬细胞相互作用以及对PSGs肺清除率的影响。将荧光标记的PSGs在体外与小鼠肺巨噬细胞或树突状细胞（DCs）±重组大鼠SP-D（rrSP-D）一起孵育。此外，在体内研究了SP-D对肺巨噬细胞和DCs摄取PSGs的影响。此外，将PSGs滴入Balb/c小鼠体内，并通过光学成像评估SP-D对肺总清除率的影响。SP-D处理在体外增加了PSG阳性巨噬细胞和DCs的数量。此外，SP-D加速了肺泡巨噬细胞的摄取/结合，并在24小时时减少了PSG阳性组织巨噬细胞和DCs的数量。然而，SP-D并不影响PSGs的肺总清除率，也不增强PSG阳性DCs诱导的T细胞增殖。总之，SP-D在体外增加了PSG阳性细胞，并在体内加速了PSG的结合/摄取。观察到的效应仅限于细胞清除机制，并不影响PSGs从肺中的总清除率。
