Luan Xi-Ying, Liu Xian-Bing
Department of Immunology, Binzhou Medical College, Yantai 264003, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Sep;26(9):849-51.
To compare and study the inhibitory effects of human bone marrow mesenchymal stem cells (HBMSCs) and human palacenta mesenchymal stem cells (HPMSCs) on T cell proliferation, and the underlying mechanism.
The expression of B7H4 on HBMSCs or the expression of PDL1 on HPMSCs were detected by FCM. Blocking experiment was used to analyze the effects of B7H4 or PDL1 on HBMSCs or HPMSCs mediating suppression on T cell proliferation and cell cycle.
FCM detection showed that HBMSCs highly expressed B7H4, while HPMSCs highly expressed PDL1, the negative immune molecules. Blockade B7H4 on HBMSCs with B7H4mAb significantly attenuated the inhibitory effects of HBMSCs on T cell proliferation. Likewise, blocking the expression of PDL1 on HPMSCs obviously weakened the suppressive effects of HPMSCs on T cell proliferation activated by PHA. Moreover, Blockade B7H4 on HBMSCs with B7H4mAb or PDL1 on HPMSCs with PDL1mAb significantly weakened the inhibitory effects of HBMSCs or HPMSCs on T cell cycle through down-regulating the cell number in G(0);/G(1); phase and up-regulating the cell number in S phase.
HBMSCs and HPMSCs could mediate the suppressive effects on T cell proliferation through expressing different negative immune molecules.
比较和研究人骨髓间充质干细胞（HBMSCs）和人胎盘间充质干细胞（HPMSCs）对T细胞增殖的抑制作用及其潜在机制。
采用流式细胞术检测HBMSCs上B7H4的表达或HPMSCs上PDL1的表达。通过阻断实验分析B7H4或PDL1对HBMSCs或HPMSCs介导的对T细胞增殖和细胞周期抑制作用的影响。
流式细胞术检测显示，HBMSCs高表达B7H4，而HPMSCs高表达负性免疫分子PDL1。用B7H4单克隆抗体阻断HBMSCs上的B7H4可显著减弱HBMSCs对T细胞增殖的抑制作用。同样，阻断HPMSCs上PDL1的表达明显减弱了HPMSCs对PHA激活的T细胞增殖的抑制作用。此外，用B7H4单克隆抗体阻断HBMSCs上的B7H4或用PDL1单克隆抗体阻断HPMSCs上的PDL1可通过下调G(0)/G(1)期细胞数量和上调S期细胞数量，显著减弱HBMSCs或HPMSCs对T细胞周期的抑制作用。
HBMSCs和HPMSCs可通过表达不同的负性免疫分子介导对T细胞增殖的抑制作用。
