Department of Immunology, Binzhou Medical University, Yantai 264003, China.
Tissue Cell. 2013 Feb;45(1):32-8. doi: 10.1016/j.tice.2012.09.002. Epub 2012 Oct 27.
Human placenta-derived mesenchymal stem cells (hPMSCs) have been shown to possess immunosuppressive effects against T cells and support the expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB). However, the characteristics of hPMSCs compared with human bone marrow-derived mesenchymal stem cells (hBMSCs) are not fully understood. Here, we show that hPMSCs have similar regulatory effects on T cell activation, proliferation and cytokine secretion as hBMSCs and demonstrate that PDL1 and B7H4, negative co-stimulatory molecules, are involved in the T cell immunosuppressive activities of hPMSCs and hBMSCs, respectively. hPMSCs efficiently enhanced the expansion of CD34⁺ cells from UCB compared with hBMSCs. Furthermore, hPMSCs maintained the expression of adhesion molecules (CD11a, CD44 and CD49e) in CD34⁺ cells. Similar effects were observed for both hPMSCs and hBMSCs on CD34⁺ cell chemotaxis and cytokine production, such as SDF-1α, IL-6 and SCF. Therefore, hPMSCs may be an ideal alternative source of hBMSCs for basic research and clinical applications, which may be significant in future efforts to explore the potential clinical utility of hPMSCs.
人胎盘间充质干细胞（hPMSCs）已被证明具有抑制 T 细胞的免疫作用，并支持从脐带血（UCB）扩增造血干细胞/祖细胞（HSPCs）。然而，hPMSCs 与人类骨髓间充质干细胞（hBMSCs）的特性尚未完全了解。在这里，我们表明 hPMSCs 对 T 细胞活化、增殖和细胞因子分泌具有相似的调节作用，并且证明 PD-L1 和 B7H4 是 hPMSCs 和 hBMSCs 分别参与 T 细胞免疫抑制活性的负共刺激分子。与 hBMSCs 相比，hPMSCs 能更有效地增强 UCB 中 CD34+细胞的扩增。此外，hPMSCs 维持 CD34+细胞中粘附分子（CD11a、CD44 和 CD49e）的表达。hPMSCs 和 hBMSCs 对 CD34+细胞趋化性和细胞因子产生（如 SDF-1α、IL-6 和 SCF）均有相似的作用。因此，hPMSCs 可能是 hBMSCs 的理想替代来源，可用于基础研究和临床应用，这可能对未来探索 hPMSCs 的潜在临床应用具有重要意义。
