[白细胞介素-2刺激的肺结核患者CD3(+) CD56(+) NKT细胞反应]

[IL-2 stimulated responses of CD3(+) CD56(+) NKT cells in pulmonary tuberculosis patients].

作者信息

Yao Chun-Yan, Wang Zhao-Hua, Jiang Li-Na, Peng Mei-Yu, Wang Jing, Li Bai-Qing

机构信息

Department of Immunology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Sep;26(9):884-6.

PMID:20815985

Abstract

AIM

To observe the activation and proliferation characteristics of IL-2 stimulated CD3(+);CD56(+); NKT cells in pulmonary tuberculosis (PTB) patients.

METHODS

Peripheral blood mononuclear cells (PBMCs) from PTB patients and normal subjects were stimulated with IL-2 and cultured for different time points. The CD69 expression on and amount of the CD3(+);CD56(+); NKT cells were detected by multi fluorescence staining and flow cytometry at different time of stimulation and culture.

RESULTS

There was no significant difference in percentage of NKT cells between PTB patients and normal healthy controls before culture. When IL-2 was used to stimulate for 0 h, 8 h, 16 h, 40 h and 64 h, the expression of CD69 on NKT cells in normal controls and PTB patients increased significantly, but the CD69 expression level of NKT cells in PTB patients was significantly higher than that in normal persons(P<0.05). In PTB patients group, PBMCs were expanded significantly after stimulated by IL-2, the absolute number of NKT cells increased from (3.44+/-1.20)x10(4); to (323.23+/-75.98) x10(4); (P<0.01), expanded by 108.69+/-59.22 fold, In normal control group the absolute number of NKT cells increased from (5.57+/-5.16)x10(4); to (1475.05+/-868.98)x10(4); (P<0.01), expanded by 246.26+/-134.06 fold, the expanding efficiency in PTB group was significantly lower than that in normal control group (P<0.05).

CONCLUSION

NKT cells in PTB patients present with high activation but low proliferation after stimulated by IL-2.

摘要

目的

观察白细胞介素-2（IL-2）刺激的肺结核（PTB）患者CD3(+)；CD56(+)；自然杀伤T（NKT）细胞的活化及增殖特性。

方法

用IL-2刺激PTB患者和正常受试者的外周血单个核细胞（PBMC），并在不同时间点进行培养。在刺激和培养的不同时间，通过多荧光染色和流式细胞术检测CD3(+)；CD56(+)；NKT细胞上CD69的表达及数量。

结果

培养前，PTB患者与正常健康对照的NKT细胞百分比无显著差异。用IL-2刺激0小时、8小时、16小时、40小时和64小时时，正常对照和PTB患者NKT细胞上CD69的表达均显著增加，但PTB患者NKT细胞的CD69表达水平显著高于正常人（P<0.05）。在PTB患者组中，PBMC经IL-2刺激后显著扩增，NKT细胞绝对数从（3.44±1.20）×10⁴增至（323.23±75.98）×10⁴（P<0.01），扩增了108.69±59.22倍；在正常对照组中，NKT细胞绝对数从（5.57±5.16）×10⁴增至（1475.05±868.98）×10⁴（P<0.01），扩增了246.26±134.06倍，PTB组的扩增效率显著低于正常对照组（P<0.05）。