通过电喷雾电离质谱（包括傅里叶变换质谱）对糖胺聚糖进行表征。
Glycosaminoglycan characterization by electrospray ionization mass spectrometry including fourier transform mass spectrometry.
作者信息
Laremore Tatiana N, Leach Franklin E, Solakyildirim Kemal, Amster I Jonathan, Linhardt Robert J
机构信息
Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, New York, USA.
出版信息
Methods Enzymol. 2010;478:79-108. doi: 10.1016/S0076-6879(10)78003-4.
Abstract
Electrospray ionization mass spectrometry (ESI MS) is a versatile analytical technique in glycomics of glycosaminoglycans (GAGs). Combined with enzymology, ESI MS is used for assessing changes in disaccharide composition of GAGs biosynthesized under different environmental or physiological conditions. ESI coupled with high-resolution mass analyzers such as a Fourier transform mass spectrometer (FTMS) permits accurate mass measurement of large oligosaccharides and intact GAGs as well as structural characterization of GAG oligosaccharides using information-rich fragmentation methods such as electron detachment dissociation. The first part of this chapter describes methods for disaccharide compositional profiling using ESI MS and the second part is dedicated to FTMS and tandem MS methods of GAG compositional and structural analysis.
摘要
电喷雾电离质谱（ESI MS）是糖胺聚糖（GAGs）糖组学中一种多功能的分析技术。结合酶学，ESI MS用于评估在不同环境或生理条件下生物合成的GAGs二糖组成的变化。ESI与高分辨率质量分析器（如傅里叶变换质谱仪（FTMS））联用，可对大的寡糖和完整的GAGs进行精确质量测量，并使用诸如电子脱离解离等信息丰富的碎裂方法对GAG寡糖进行结构表征。本章的第一部分描述了使用ESI MS进行二糖组成分析的方法，第二部分专门介绍了用于GAG组成和结构分析的FTMS和串联质谱方法。
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