Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Anal Biochem. 2010 Jun 15;401(2):236-41. doi: 10.1016/j.ab.2010.03.004. Epub 2010 Mar 6.
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
使用市售设备进行连续洗脱聚丙烯酰胺凝胶电泳（CE-PAGE），可在 6 小时内分离聚合度为 4 至 32 的毫克级肝素寡糖。通过强阴离子交换高效液相色谱、电喷雾电离傅里叶变换质谱和二维核磁共振波谱，可确证 CE-PAGE 分离的寡糖的纯度和结构完整性。所描述的方法简单直接，用时效率高，可得到大小均一的寡糖，可用于测序、蛋白质结合和其他结构-功能关系研究。
