Yin Jian, Seeberger Peter H
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Research Campus Potsdam-Golm, Potsdam, Germany.
Methods Enzymol. 2010;478:197-218. doi: 10.1016/S0076-6879(10)78009-5.
Carbohydrate microarrays have become crucial tools for revealing the biological interactions and functions of glycans, primarily because the microarray format enables the investigation of large numbers of carbohydrates at a time. Heparan sulfate (HS) and heparin are the most structurally complex glycosaminoglycans (GAGs). In this chapter, we describe the preparation of a small library of HS/heparin oligosaccharides, and the fabrication of HS/heparin microarrays that have been used to establish HS/heparin-binding profiles. Fibroblast growth factors (FGFs), natural cytotoxicity receptors (NCRs), and chemokines were screened to illuminate the very important biological functions of these glycans.
碳水化合物微阵列已成为揭示聚糖的生物相互作用和功能的关键工具，主要是因为微阵列形式能够一次研究大量碳水化合物。硫酸乙酰肝素（HS）和肝素是结构最复杂的糖胺聚糖（GAG）。在本章中，我们描述了一个小型硫酸乙酰肝素/肝素寡糖文库的制备，以及用于建立硫酸乙酰肝素/肝素结合图谱的硫酸乙酰肝素/肝素微阵列的制作。对成纤维细胞生长因子（FGF）、天然细胞毒性受体（NCR）和趋化因子进行了筛选，以阐明这些聚糖非常重要的生物学功能。
