O'Reilly Mary K, Paulson James C
Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.
Methods Enzymol. 2010;478:343-63. doi: 10.1016/S0076-6879(10)78017-4.
Siglecs have emerged as an important family of immunomodulatory glycan-binding proteins that can bind sialoside ligands both on the same cell surface, in cis, and on other cells, in trans. Expression of siglecs varies among a variety of immune cells, and tools to probe siglecs on these cells are crucial to understanding their function. In designing synthetic ligands, competition by cis ligands requires the use of multivalency to achieve sufficient avidity to stably bind siglecs on native cells. This chapter describes the use of multivalent ligands to probe cell surfaces, as well as to investigate ligand binding to recombinant siglecs.
唾液酸结合免疫球蛋白样凝集素（Siglecs）已成为一类重要的免疫调节性聚糖结合蛋白家族，它们既能在同一细胞表面以顺式结合唾液酸配体，也能在其他细胞上以反式结合唾液酸配体。Siglecs在多种免疫细胞中的表达各不相同，而用于探测这些细胞上Siglecs的工具对于理解其功能至关重要。在设计合成配体时，顺式配体的竞争作用要求使用多价性来获得足够的亲和力，以便在天然细胞上稳定结合Siglecs。本章描述了使用多价配体探测细胞表面，以及研究配体与重组Siglecs结合的方法。
