Soler C, Blázquez C, Sánchez N, Cervelló M, Sánchez P, Núñez A
Departament de Biologia Animal (Secció Fisiologia), Facultat de Ciències Biològiques, Universitat de València, Burjassot, Spain.
J Anat. 1990 Aug;171:147-53.
Doses (2.5 mg/kg) of rhodamine 6GO were given subcutaneously to five Charles-Rivel mice. After 15 minutes the testis and epididymis were observed with a fluoroscope and a fluorescent microscope which showed that rhodamine 6GO was present in a rhodamine-fluorescent zone (RF-zone) of the caput epididymidis. In this RF-zone, the fluorochrome was localised in the epithelial cells of the epididymal duct but not in the lumen nor the vascular system. At the edge of the RF-zone a wide band of connective tissue was present; the epithelium of the epididymal duct in this zone was higher and its tubular diameter greater than in the rest of the epididymal duct and spermatozoa were usually not present in the lumen. A possible role played by the epididymis in the endocrine control of the testicular function is suggested.
给5只查尔斯 - 里韦尔小鼠皮下注射剂量为2.5毫克/千克的罗丹明6G。15分钟后，用荧光镜和荧光显微镜观察睾丸和附睾，结果显示罗丹明6G存在于附睾头的罗丹明荧光区（RF区）。在这个RF区，荧光染料定位于附睾管的上皮细胞中，而不是在管腔或血管系统中。在RF区边缘有一条宽的结缔组织带；该区域附睾管的上皮更高，其管径比附睾管其他部分更大，管腔内通常不存在精子。提示附睾在睾丸功能的内分泌控制中可能发挥作用。
