Olsson J H, Granberg S
Department of Obstetrics and Gynaecology, University of Göteborg, Sweden.
Hum Reprod. 1990 Nov;5(8):928-32. doi: 10.1093/oxfordjournals.humrep.a137222.
The effects of en-clomiphene and zu-clomiphene (10(-7) - 10(-5) M) on oestradiol synthesis in human granulosa cells were studied. Granulosa cells were obtained from stimulated cycles in women undergoing oocyte retrieval, in the course of in-vitro fertilization/embryo transfer due to mechanical infertility. En-clomiphene and zu-clomiphene (10(-5) M) significantly reduced basal and LH-stimulated oestradiol synthesis after 2-4 days of culture. The LH-stimulated synthesis was also significantly and reversibly reduced by zu-clomiphene at lower concentrations (10(-6) - 10(-7) M) and by a mixture of en- and zu-clomiphene (10(-6) M). It is concluded that both the mixture of en- and zu-clomiphene and the isomers alone have direct inhibitory effects on oestradiol synthesis in human granulosa cells in vitro. Furthermore, it cannot be excluded that the LH-stimulated oestradiol synthesis is inhibited by zu-clomiphene at concentrations found in follicular fluid after administration of clomiphene citrate.
研究了恩氯米芬和左氯米芬（10⁻⁷ - 10⁻⁵M）对人颗粒细胞雌二醇合成的影响。颗粒细胞取自因机械性不育而接受体外受精/胚胎移植的女性在促排卵周期中进行卵母细胞采集时。培养2 - 4天后，恩氯米芬和左氯米芬（10⁻⁵M）显著降低基础和促黄体生成素（LH）刺激的雌二醇合成。在较低浓度（10⁻⁶ - 10⁻⁷M）时，左氯米芬以及恩氯米芬和左氯米芬的混合物（10⁻⁶M）也能显著且可逆地降低LH刺激的合成。得出的结论是，恩氯米芬和左氯米芬的混合物以及单独的异构体在体外对人颗粒细胞的雌二醇合成均有直接抑制作用。此外，不能排除在给予枸橼酸氯米芬后，卵泡液中发现的左氯米芬浓度会抑制LH刺激的雌二醇合成。
