Delbosc B, Urlacher A, Tongio M M, Flament J
Clinique ophtalmologique, Hospices civils, Strasbourg.
J Fr Ophtalmol. 1990;13(11-12):542-6.
To increase the number of HLA typed corneas a microlymphocytotoxicity assay on lymphocytes and PHA lymphoblasts was investigated. A double immunofluorescence technique using magnetic beads coated with anti-T8 (for class I) and anti-DR (for class II) monomorphic antibodies was applied. Blood samples from 50 non selected donors were obtained. HLA class I and class II typing was possible in 74% of the cases. Using PBL's (Peripheral Blood Lymphocytes) HLA class I could be defined in 29 out of 50 Cases and class II in 15 out of 50 cases. On PHA blasts HLA class I and class II antigens could be defined in 32 and 33 out of 50 cases respectively. Mean time of culture was 10 days (6-20). No influence of donor age and post partum time could be observed. By combination of both methods a significant proportion of eye donors could be reliably typed within a short time.
为了增加HLA分型角膜的数量，对淋巴细胞和PHA淋巴细胞母细胞进行了微量淋巴细胞毒性试验。应用了一种双重免疫荧光技术，该技术使用包被有抗T8（用于I类）和抗DR（用于II类）单态抗体的磁珠。采集了50名未经过筛选的供体的血样。74%的病例能够进行HLA I类和II类分型。使用外周血淋巴细胞（PBL），50例中有29例可确定HLA I类，50例中有15例可确定II类。对于PHA淋巴细胞母细胞，50例中分别有32例和33例可确定HLA I类和II类抗原。平均培养时间为10天（6 - 20天）。未观察到供体年龄和产后时间的影响。通过两种方法的结合，相当比例的眼库供体能够在短时间内可靠地分型。
