Hirose M, Shinohara K, Watanabe H, Lee E, Kuroda Y
Division of Transfusion Medicine, School of Medicine, University of Tokushima, Japan.
Tokushima J Exp Med. 1996 Dec;43(3-4):87-91.
The use of phytohemagglutinin (PHA)/interleukin-2 (IL-2) activated lymphocytes for HLA typing and the comparison with those of fresh peripheral blood lymphocytes are described. For detecting class 1 HLA antigens, PHA/IL-2 activated lymphocytes showed higher sensitivity than fresh lymphocytes of the peripheral blood in serological microcytotoxicity testing method. Their reaction specificity for the panel of alloantisera for class 1 HLA antigens was the same. On the contrary, for detecting class 2 HLA antigens, PHA/IL-2 activated lymphocytes showed lower sensitivity than those with fresh B-cells. For class 2 HLA typing, the typing with DNA methods showed the best results. Thus, we conclude that a combination of a serological typing method using PHA/IL-2 activated lymphocytes for class 1 HLA antigens with a DNA method for typing class 2 HLA antigens currently offers the best approach for the detection of HLA antigens.
本文描述了使用植物血凝素（PHA）/白细胞介素-2（IL-2）激活的淋巴细胞进行HLA分型，并与新鲜外周血淋巴细胞进行比较。在血清学微量细胞毒性检测方法中，用于检测Ⅰ类HLA抗原时，PHA/IL-2激活的淋巴细胞比外周血新鲜淋巴细胞表现出更高的敏感性。它们对Ⅰ类HLA抗原同种异体抗血清组的反应特异性相同。相反，在检测Ⅱ类HLA抗原时，PHA/IL-2激活的淋巴细胞比新鲜B细胞表现出更低的敏感性。对于Ⅱ类HLA分型，DNA方法分型显示出最佳结果。因此，我们得出结论，目前使用PHA/IL-2激活的淋巴细胞进行Ⅰ类HLA抗原血清学分型方法与DNA方法进行Ⅱ类HLA抗原分型相结合，为检测HLA抗原提供了最佳方法。
