Adewola A, Mage R, Hansen M, Barbaro B, Mendoza-Elias J, Harvat T, Morel P H, Oberholzer J, Wang Y
Department of Transplant/Surgery, University of Illinois, Chicago, IL, USA.
Cryo Letters. 2010 Jul-Aug;31(4):310-7.
Two different approaches of controlled cooling of the COBE 2991 cell-separator for islet purification were evaluated. The first method is the new Geneva COBE cooling system (GCCS), which consists of an electronically controlled liquid nitrogen injection system. The second is the University of Illinois at Chicago cooling system (UICCS), which consists of a specially designed "Cold Room" maintained at 1-8 C. For the GCCS, the mean temperatures of the gradient solutions were measured at the beginning and end of centrifugation were found to be 7 +/-0.7 C and 6.8 +/-0.6 C respectively. For the UICCS, the mean temperature of the gradients at the beginning and end of centrifugation were 4.7 +/-0.53 C and 7.03 C+/-0.91 C respectively. The presented COBE cooling systems can easily be adapted to a COBE 2991 cell-separator and are efficient in maintaining gradient solutions at a defined low temperature during centrifugation.
评估了用于胰岛纯化的COBE 2991细胞分离器的两种不同的控制冷却方法。第一种方法是新的日内瓦COBE冷却系统（GCCS），它由一个电子控制的液氮注入系统组成。第二种是伊利诺伊大学芝加哥分校冷却系统（UICCS），它由一个维持在1-8摄氏度的特殊设计的“冷藏室”组成。对于GCCS，在离心开始和结束时测量的梯度溶液的平均温度分别为7±0.7摄氏度和6.8±0.6摄氏度。对于UICCS，离心开始和结束时梯度的平均温度分别为4.7±0.53摄氏度和7.03±0.91摄氏度。所介绍的COBE冷却系统可以很容易地适配到COBE 2991细胞分离器上，并且在离心过程中能有效地将梯度溶液维持在规定的低温。
