Buenos Aires Province Tuberculosis Control Program Reference Laboratory, Dr Cetrángolo Hospital, Buenos Aires, Argentina.
Int J Tuberc Lung Dis. 2010 Sep;14(9):1169-75.
Dr Cetrángolo Hospital, Buenos Aires Province, Argentina.
Evaluation of the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system and the colorimetric-based method (CMM) for first- and second-line drug susceptibility testing (FL-DST, SL-DST) against Mycobacterium tuberculosis.
FL-DST was studied using SIRE MGIT 960. Minimal inhibitory concentrations (MICs) for isoniazid (INH), streptomycin, rifampicin (RMP), ethambutol (EMB) and levofloxacin (LVX) were also determined by CMM using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT). MICs for amikacin (AMK), kanamycin (KM), capreomycin (CPM), ethionamide (ETH), cycloserine, ofloxacin (OFX), linezolide (LZ) and moxifloxacin (MFX) were determined on 94 multidrug-resistant M. tuberculosis isolates by MGIT 960 and CMM. Statistical methods were applied to define drug-susceptible and drug-resistant isolates on the basis of the comparison between results obtained by gold standards.
A total of 1626 clinical isolates were studied. Critical drug concentrations could be defined in less than 10 days for both CMM and MGIT 960. CMM was cheaper but more laborious than MGIT 960. The highest performances of both methods were achieved for AMK, RMP, OFX, LZ and MFX, followed by INH, ETH, KM, CPM and LVX (tested only by CMM).
Both methods could be implemented as rapid diagnostic tools to detect drug-resistant isolates in clinical practice.
阿根廷布宜诺斯艾利斯省的 Cetrángolo 医院。
评估 BACTEC 分枝杆菌生长指示管（MGIT）960 系统和比色法（CMM）用于结核分枝杆菌一线和二线药物敏感性测试（FL-DST、SL-DST）的效果。
使用 SIRE MGIT 960 进行 FL-DST 研究。使用 3-（4,5-二甲基噻唑基-2）-2,5-二苯基四氮唑溴盐（MTT）通过 CMM 还确定了异烟肼（INH）、链霉素、利福平（RMP）、乙胺丁醇（EMB）和左氧氟沙星（LVX）的最小抑菌浓度（MIC）。通过 MGIT 960 和 CMM 确定了 1626 株耐多药结核分枝杆菌分离株中阿米卡星（AMK）、卡那霉素（KM）、卷曲霉素（CPM）、乙硫异烟胺（ETH）、环丝氨酸、氧氟沙星（OFX）、利奈唑胺（LZ）和莫西沙星（MFX）的临界药物浓度。应用统计学方法，根据金标准比较结果，定义了药敏和耐药分离株。
共研究了 1626 株临床分离株。CMM 和 MGIT 960 均能在不到 10 天内确定临界药物浓度。CMM 比 MGIT 960 更便宜，但更繁琐。两种方法的最高性能均适用于 AMK、RMP、OFX、LZ 和 MFX，其次是 INH、ETH、KM、CPM 和 LVX（仅通过 CMM 测试）。
两种方法都可以作为快速诊断工具，用于在临床实践中检测耐药分离株。
