Okinawa Prefectural Institute Health and Environment, 2085 Ozato, Nanjo-shi Okinawa 901-1202, Japan.
J Food Prot. 2010 Aug;73(8):1529-32. doi: 10.4315/0362-028x-73.8.1529.
The enumeration and evaluation of the activity of marine bacteria are important in the food industry. However, detection of marine bacteria in seawater or seafood has not been easy. The Petrifilm aerobic count plate (ACP) is a ready-to-use alternative to the traditional enumeration media used for bacteria associated with food. The purpose of this study was to evaluate the usefulness of a simple detection and enumeration method utilizing the Petrifilm ACP for enumeration of aerobic marine bacteria from seawater and an edible seaweed, Caulerpa lentillifera. The efficiency of enumeration of total aerobic marine bacteria on Petrifilm ACP was compared with that using the spread plate method on marine agar with 80 seawater and 64 C. lentillifera samples. With sterile seawater as the diluent, a close correlation was observed between the method utilizing Petrifilm ACP and that utilizing the conventional marine agar (r=0.98 for seawater and 0.91 for C. lentillifera). The Petrifilm ACP method was simpler and less time-consuming than the conventional method. These results indicate that Petrifilm ACP is a suitable alternative to conventional marine agar for enumeration of marine microorganisms in seawater and C. lentillifera samples.
海洋细菌的计数和评估在食品工业中很重要。然而，在海水中或海鲜中检测海洋细菌并不容易。Petrifilm 需氧计数平板（ACP）是一种替代传统用于与食物相关细菌的计数培养基的即用型方法。本研究的目的是评估利用 Petrifilm ACP 进行简单检测和计数的方法，从海水中和可食用海藻龙须菜（Caulerpa lentillifera）中计数需氧海洋细菌的有用性。在 80 个海水和 64 个龙须菜样本中，将 Petrifilm ACP 上总需氧海洋细菌的计数效率与在海洋琼脂上的平板涂布法进行了比较。用无菌海水作为稀释剂，利用 Petrifilm ACP 的方法与传统的海洋琼脂法之间存在密切相关性（海水为 r=0.98，龙须菜为 r=0.91）。Petrifilm ACP 方法比传统方法更简单，耗时更少。这些结果表明，Petrifilm ACP 是替代传统海洋琼脂用于海水和龙须菜样本中海洋微生物计数的合适方法。
