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评估用于多种食品中需氧菌群和大肠菌群计数的Petrifilm方法。

Evaluation of Petrifilm methods for enumeration of aerobic flora and coliforms in a wide range of foods.

作者信息

Blackburn C W, Baylis C L, Petitt S B

机构信息

Department of Food Microbiology, Leatherhead Food Research Association, Surrey, UK.

出版信息

Lett Appl Microbiol. 1996 Feb;22(2):137-40. doi: 10.1111/j.1472-765x.1996.tb01127.x.

DOI:10.1111/j.1472-765x.1996.tb01127.x
PMID:8936373
Abstract

Petrifilm is a ready-to-use alternative to traditional microbial enumeration methods. The Petrifilm Aerobic Count Plate (ACP) and Coliform Count Plate (CCP) were compared with standard methods for the enumeration of the aerobic mesophilic flora and coliform bacteria in 91 foods covering a wide range of different food commodities. There was good correlation between the Petrifilm ACP and the standard aerobic colony count method (r = 0.989) and between the Petrifilm CCP and the standard Violet Red Bile Agar plating method (r = 0.872). In both cases, the Petrifilm methods had a better repeatability than the standard methods. The Petrifilm ACP and CCP were shown to be practical and accurate alternatives to standard enumeration methods in a wide range of foods, with benefits of saving time, labour and incubator space.

摘要

Petrifilm是一种可直接使用的传统微生物计数方法的替代方法。将Petrifilm需氧菌计数平板(ACP)和大肠菌群计数平板(CCP)与标准方法进行比较,以对91种涵盖广泛不同食品种类的食品中的嗜温需氧菌群和大肠菌群进行计数。Petrifilm ACP与标准需氧菌落计数法之间(r = 0.989)以及Petrifilm CCP与标准紫红胆盐琼脂平板计数法之间(r = 0.872)具有良好的相关性。在这两种情况下,Petrifilm方法都比标准方法具有更好的重复性。结果表明,在广泛的食品中,Petrifilm ACP和CCP是标准计数方法的实用且准确的替代方法,具有节省时间、人力和培养箱空间的优点。

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