Utsunomiya N, Chuyen N V, Kato H
Department of Food Science, Kyoritsu Women's University, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1990 Aug;36(4):387-97. doi: 10.3177/jnsv.36.4-supplementi_387.
Casein was modified by glucose, diacetyl, or hexanal at 50 degrees C, RH 75% for 1, 7, or 11 days. The chemical changes and digestibility in vitro of these nondialyzable caseins were investigated. The effects of these nondialyzable caseins supplemented with lost amino acids, on rats were studied by pair-feeding for 2 months. It was observed that internal organs such as liver, spleen, kidney, stomach, small intestine, cecum, colon and rectum were mostly unchanged. Biochemical values such as hematocrit, cholesterol, triglyceride, GPT, and GOT were also unchanged. However, the quantity of leucocytes was increased and serum glucose was decreased by feeding rats with modified caseins. Significant decrease in weight gain of rats fed with modified casein was observed, and the rate of decrease depended on the degree of modification of casein by carbonyl compounds. From these results, we supported the suggestion that some inhibitory or antinutritional compounds might be formed during the modification of casein by carbonyl compounds.
酪蛋白在50摄氏度、相对湿度75%的条件下分别用葡萄糖、双乙酰或己醛处理1天、7天或11天。研究了这些不可透析酪蛋白的化学变化及体外消化率。通过对大鼠进行为期2个月的配对饲养，研究了补充缺失氨基酸的这些不可透析酪蛋白对大鼠的影响。观察到肝脏、脾脏、肾脏、胃、小肠、盲肠、结肠和直肠等内脏器官大多未发生变化。血细胞比容、胆固醇、甘油三酯、谷丙转氨酶和谷草转氨酶等生化指标也未改变。然而，给大鼠喂食改性酪蛋白后，白细胞数量增加，血糖降低。观察到喂食改性酪蛋白的大鼠体重增加显著下降，下降速率取决于羰基化合物对酪蛋白的改性程度。从这些结果来看，我们支持这样的观点，即羰基化合物对酪蛋白进行改性的过程中可能会形成一些抑制性或抗营养性化合物。
