Ohtani H, Matoba K, Saika Y, Tanaka R, Yamada Y, Bando K, Takahashi T, Mune M, Yukawa S, Nomoto H
Third Department of Internal Medicine, Wakayama Medical University, Japan.
Nihon Jinzo Gakkai Shi. 1990 Nov;32(11):1145-52.
In an attempt to elucidate the relationship between the progress of glomerular injury and abnormalities of lipid metabolism, we investigated glomerular deposition of apolipoprotein B (apo B) in renal biopsy specimens from 60 patients with glomerular diseases by indirect immunofluorescence using antihuman apo B-100 monoclonal antibody in comparison with clinical and histopathological findings. The patients were divided into 2 groups according to the intensity in staining of apo B in glomerulus (group A: negative or weakly positive; and group B: definitely positive). Staining of apo B in glomerulus was found in 37 patients (62%). The levels of serum total cholesterol, phospholipids, low density lipoprotein cholesterol and apo B in group B were significantly higher than in group A. The urinary protein excretion in group B was greater than that in group A. Group B was also shown to have a significantly decrease in renal function. Light microscopy revealed severe mesangial proliferation in patients with IgA nephropathy of group B. These findings suggested that glomerular apo B containing-lipoprotein deposition may play an important role in the progression of glomerular injury.
为了阐明肾小球损伤进展与脂质代谢异常之间的关系，我们使用抗人载脂蛋白B-100单克隆抗体，通过间接免疫荧光法研究了60例肾小球疾病患者肾活检标本中载脂蛋白B（apo B）的肾小球沉积情况，并与临床和组织病理学结果进行了比较。根据肾小球中apo B染色强度，将患者分为2组（A组：阴性或弱阳性；B组：阳性）。37例患者（62%）肾小球中有apo B染色。B组血清总胆固醇、磷脂、低密度脂蛋白胆固醇和apo B水平显著高于A组。B组尿蛋白排泄量大于A组。B组肾功能也显著下降。光镜检查显示B组IgA肾病患者系膜重度增生。这些发现提示含apo B的肾小球脂蛋白沉积可能在肾小球损伤进展中起重要作用。
