Unidade de Bioenergia, LNEG-Laboratório Nacional de Energia e Geologia, Estrada do Paço do Lumiar, 22, 1649-038, Lisbon, Portugal.
J Ind Microbiol Biotechnol. 2011 Jan;38(1):221-7. doi: 10.1007/s10295-010-0823-5. Epub 2010 Sep 5.
Detailed kinetic and physiological characterisation of eight mannitol-producing lactic acid bacteria, Leuconostoc citreum ATCC 49370, L. mesenteroides subsp. cremoris ATCC19254, L. mesenteroides subsp. dextranicum ATCC 19255, L. ficulneum NRRL B-23447, L. fructosum NRRL B-2041, L. lactis ATCC 19256, Lactobacillus intermedius NRRL 3692 and Lb. reuteri DSM 20016, was performed using a carob-based culture medium, to evaluate their different metabolic capabilities. Cultures were thoroughly followed for 30 h to evaluate consumption of sugars, as well as production of biomass and metabolites. All strains produced mannitol at high yields (>0.70 g mannitol/g fructose) and volumetric productivities (>1.31 g/l h), and consumed fructose and glucose simultaneously, but fructose assimilation rate was always higher. The results obtained enable the studied strains to be divided mainly into two groups: one for which glucose assimilation rates were below 0.78 g/l h (strains ATCC 49370, ATCC 19256 and ATCC 19254) and the other for which they ranged between 1.41 and 1.89 g/l h (strains NRRL B-3692, NRRL B-2041, NRRL B-23447 and DSM 20016). These groups also exhibited different mannitol production rates and yields, being higher for the strains with faster glucose assimilation. Besides mannitol, all strains also produced lactic acid and acetic acid. The best performance was obtained for L. fructosum NRRL B-2041, with maximum volumetric productivity of 2.36 g/l h and the highest yield, stoichiometric conversion of fructose to mannitol.
对八种甘露醇生产乳酸菌，即肠膜明串珠菌 ATCC 49370、乳脂明串珠菌亚种。乳脂亚种 ATCC19254、乳脂明串珠菌亚种。右旋糖明串珠菌 ATCC 19255、纤维二糖明串珠菌 NRRL B-23447、果糖明串珠菌 NRRL B-2041、乳链球菌 ATCC 19256、中间乳杆菌 NRRL 3692 和雷特氏乳杆菌 DSM 20016 的详细动力学和生理学特性进行了研究，使用角豆基培养基进行了评价，以评估它们不同的代谢能力。在 30 小时内对培养物进行了彻底跟踪，以评估糖的消耗情况，以及生物量和代谢物的产生情况。所有菌株都以高产量（>0.70 g 甘露醇/g 果糖）和体积产率（>1.31 g/l h）生产甘露醇，并同时消耗果糖和葡萄糖，但果糖的同化率始终较高。所得结果可将研究菌株主要分为两组：一组的葡萄糖同化率低于 0.78 g/l h（菌株 ATCC 49370、ATCC 19256 和 ATCC 19254），另一组的葡萄糖同化率在 1.41 至 1.89 g/l h 之间（菌株 NRRL B-3692、NRRL B-2041、NRRL B-23447 和 DSM 20016）。这两组菌株的甘露醇生产速率和产量也不同，葡萄糖同化率较高的菌株产量较高。除甘露醇外，所有菌株还产生乳酸和乙酸。L. fructosum NRRL B-2041 的表现最佳，其最大体积产率为 2.36 g/l h，果糖到甘露醇的转化率最高。
