Liu Jing, Ding Guangzhi, Yu Shishan
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2010 Jun;35(11):1421-4.
The chemical constituents from the stem barks of Vernonia cumingiana were investigated. Various chromatographic techniques such as silica gel chromatography, Sephadex LH-20, ODS column chromatography and HPLC were used to isolate and purify the constituents. The structures were elucidated by spectral methods. Twelve compounds were isolated from the 95% ethanol extract and their structures were elucidated as methyl 3,5-dicaffeoylquinate (1), methyl 3,4-dicaffeoylquinate (2), ethyl 3,4-dicaffeoylquinate (3), methyl 3,4,5-tricaffeoylquinate (4), stigmasterol (5), alpha-spinasterol (6), beta-sitosterol (7), 24-methylene-lanosta-9 (11)-en-3beta-acetate (8), ethyl gallate (9), di-n-butyl-phthalate (10), stearic acid (11) and palmitic acid (12). Compounds 1-12 were isolated from this plant for the first time.
对聚花斑鸠菊茎皮的化学成分进行了研究。采用硅胶柱色谱、葡聚糖凝胶LH-20、ODS柱色谱和高效液相色谱等多种色谱技术对其成分进行分离和纯化。通过光谱方法对结构进行了鉴定。从95%乙醇提取物中分离出12种化合物，其结构鉴定为3,5-二咖啡酰奎宁酸甲酯（1）、3,4-二咖啡酰奎宁酸甲酯（2）、3,4-二咖啡酰奎宁酸乙酯（3）、3,4,5-三咖啡酰奎宁酸甲酯（4）、豆甾醇（5）、α-菠菜甾醇（6）、β-谷甾醇（7）、24-亚甲基-羊毛甾-9(11)-烯-3β-乙酸酯（8）、没食子酸乙酯（9）、邻苯二甲酸二正丁酯（10）、硬脂酸（11）和棕榈酸（12）。化合物1-12均为首次从该植物中分离得到。
