鹅细小病毒感染在免疫系统器官中的靶细胞鉴定

Identification of target cells for Goose parvovirus infection in the immune system organs.

作者信息

Zhu L Q, Ding X Y, Tao J, Wang J Y, Zhang X J, Wang X B, Hu Y, Li H F, Chen K W, Zhu G Q

机构信息

College of Veterinary Medicine, Yangzhou University, Yangzhou, P. R. China.

出版信息

Acta Virol. 2010;54(3):211-5. doi: 10.4149/av_2010_03_211.

DOI:10.4149/av_2010_03_211

PMID:20822314

Abstract

Target cells for Goose parvovirus (GPV) in natural infection are still unknown. In this study, immune system organs namely the spleen, bone marrow, thymus, bursa of Fabricius, and blood of experimentally GPV-infected goslings were examined by an immunoassay and flow cytometry for the presence of viral antigen and by a PCR for viral genome. The results indicated that the virus replicated in some cells of the spleen and bone marrow, but not in peripheral blood lymphocytes (PBLs). These data suggested that some cell populations in the spleen and bone marrow were targets for GPV infection. In addition, the immunoassay used for the detection of GPV was found comparable with a PCR in reliability and sensitivity.

摘要

鹅细小病毒（GPV）自然感染的靶细胞仍不清楚。在本研究中，通过免疫测定和流式细胞术检测实验性感染GPV的雏鹅的免疫系统器官，即脾脏、骨髓、胸腺、法氏囊和血液中病毒抗原的存在情况，并通过PCR检测病毒基因组。结果表明，该病毒在脾脏和骨髓的一些细胞中复制，但在外周血淋巴细胞（PBL）中不复制。这些数据表明，脾脏和骨髓中的一些细胞群体是GPV感染的靶细胞。此外，发现用于检测GPV的免疫测定在可靠性和灵敏度方面与PCR相当。