中国鹅（Anser cygnoides）CD8α在小鹅瘟病毒（NGVEV）和鹅细小病毒（GPV）感染期间的免疫生物学活性及抗病毒调控作用

Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection.

作者信息

Chen Shun, Zhao Qiurong, Qi Yulin, Liu Fei, Wang Mingshu, Jia Renyong, Zhu Dekang, Liu Mafeng, Chen Xiaoyue, Cheng Anchun

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, 46 Xinkang Road, Ya'an, Sichuan 625014, China Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China These authors contributed equally as co-first authors of this work.

出版信息

Poult Sci. 2015 Jan;94(1):17-24. doi: 10.3382/ps/peu024.

DOI:10.3382/ps/peu024

PMID:25577792

Abstract

The CD8 molecule is a cell membrane glycoprotein expressed on cytotoxic T lymphocytes, which are involved in the clearance of viruses. However, the functional characterization of goose CD8α is still unclear. The immunobiological characterization of goose CD8α in goose spleen mononuclear cells (MNCs) was examined by real-time quantitative PCR (qPCR). It was shown that CD8α mRNA levels were significantly up-regulated by in vitro treatment of MNCs with phytohemagglutinin (PHA), concanavalin A (ConA), and polyinosinic-polycytidylic acid (poly I:C) in a dose-dependent way, but lipopolysaccharides (LPSs) did not have this same effect. Moreover, the time-course effect of CD8α expression in response to mitogens (PHA, ConA, and poly I:C) was evaluated in MNCs. A significant increase in the transcriptional levels of CD8α was detected in new type gosling viral enteritis virus (NGVEV)-infected goose MNCs at 48 h postinfection (PI) and in goose parvovirus (GPV)-infected MNCs at 72 h PI. Also, the number of CD8α+ cells was significantly increased during viral infection from 72 h on. The seminal changes in mRNA profiles of antiviral cytokines (IFN-α, IFN-γ, and IL-18) were observed and were significantly increased during late phases of NGVEV and GPV infection. Accordingly, our data not only contribute to the understanding of the immune characteristics of goose CD8α, but they also provide new insight into the innate antiviral immunity of geese.

摘要

CD8分子是一种细胞膜糖蛋白，表达于细胞毒性T淋巴细胞上，这些细胞参与病毒清除。然而，鹅CD8α的功能特性仍不清楚。通过实时定量PCR（qPCR）检测了鹅脾脏单核细胞（MNCs）中鹅CD8α的免疫生物学特性。结果表明，用植物血凝素（PHA）、刀豆球蛋白A（ConA）和聚肌苷酸-聚胞苷酸（poly I:C）体外处理MNCs后，CD8α mRNA水平呈剂量依赖性显著上调，但脂多糖（LPSs）没有相同作用。此外，还评估了MNCs中CD8α表达对丝裂原（PHA、ConA和poly I:C）的时间进程效应。在感染新型雏鹅病毒性肠炎病毒（NGVEV）的鹅MNCs感染后48小时（PI）以及感染鹅细小病毒（GPV）的MNCs感染后72小时PI，检测到CD8α转录水平显著增加。而且，从72小时起病毒感染期间CD8α+细胞数量显著增加。观察到抗病毒细胞因子（IFN-α、IFN-γ和IL-18）mRNA谱的显著变化，并且在NGVEV和GPV感染后期显著增加。因此，我们的数据不仅有助于理解鹅CD8α的免疫特性，还为鹅的先天性抗病毒免疫提供了新的见解。