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建立一种用于检测具有短喙和矮小综合征病鸭或死鸭中经典鹅细小病毒和新型鹅细小病毒相关病毒的双重半巢式 PCR 检测方法。

Development of a duplex semi-nested PCR assay for detection of classical goose parvovirus and novel goose parvovirus-related virus in sick or dead ducks with short beak and dwarfism syndrome.

机构信息

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 201718 Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, 201718 Tai'an, China.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, 201718 Tai'an, China.

出版信息

J Virol Methods. 2017 Nov;249:165-169. doi: 10.1016/j.jviromet.2017.09.011. Epub 2017 Sep 14.

Abstract

Duck short beak and dwarfism syndrome (SBDS) is an emerging infectious disease caused by a novel goose parvovirus-related virus (NGPV) in China. Until now, it remains uncertain whether the Cherry Valley ducks and mule ducks with SBDS are co-infected with classical goose parvovirus (GPV) and NGPV. In this study, a duplex semi-nested PCR assay with high specificity and sensitivity was developed for detection of the two viruses. Using the duplex PCR assay, NGPV was tested positive in all the 15 duck flocks with SBDS, whereas classical GPV was not detected in all the 133 sick and dead ducks collected from East China. A total of 87 (91.58%) Cherry Valley ducks aged from 5 to 18days and 35 (92.11%) mule ducks aged from 17 to 25days were detected positive for NGPV. In the NGPV-positive ducks, the virus detection rates were 81.97% to 8.20% in heart, liver, spleen, lung, kidney, pancreas, bile, thymus, bursa of Fabricius, and brain. The results indicated that NGPV was prevalent in the duck flocks of East China, whereas classical GPV was not detected in Cherry Valley ducks and mule ducks with SBDS. NGPV has extensive tissue tropism in Cherry Valley duck and mule duck, which could invade both the central and peripheral immune organs and break through the blood-brain barrier of ducks.

摘要

鸭短喙侏儒综合征(SBDS)是一种在中国由新型鹅细小病毒相关病毒(NGPV)引起的新发传染病。到目前为止,SBDS 樱桃谷鸭和骡鸭是否同时感染经典鹅细小病毒(GPV)和 NGPV 尚不确定。本研究建立了一种具有高特异性和灵敏度的双重半巢式 PCR 检测方法,用于检测两种病毒。使用双重 PCR 检测方法,在所有 15 个 SBDS 鸭群中均检测到 NGPV 阳性,而在华东地区采集的 133 只病死鸭中均未检测到经典 GPV。总共检测到 87 只(91.58%)5-18 日龄樱桃谷鸭和 35 只(92.11%)17-25 日龄骡鸭的 NGPV 阳性。在 NGPV 阳性鸭中,病毒在心脏、肝脏、脾脏、肺脏、肾脏、胰腺、胆汁、胸腺、法氏囊和大脑中的检出率为 81.97%至 8.20%。结果表明,NGPV 在华东鸭群中流行,而 SBDS 樱桃谷鸭和骡鸭中未检测到经典 GPV。NGPV 在樱桃谷鸭和骡鸭中有广泛的组织嗜性,可侵袭中枢和外周免疫器官,并突破鸭的血脑屏障。

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