Research Institute, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba 275-0016, Japan.
Virology. 2010 Nov 10;407(1):152-9. doi: 10.1016/j.virol.2010.08.011. Epub 2010 Sep 6.
The effect of lipolysis by lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) on hepatitis C virus (HCV) infection was evaluated. First, medium from HuH7.5 cells bearing HCV genome replication was treated with LPL. LPL treatment led to reduced HCV infectivity, shifted HCV to higher densities, and lowered the amount of apolipoprotein E-associated HCV. The effect of endogenous HTGL secreted from HuH7.5 on HCV infectivity was next examined. Neutralization of HTGL by an anti-HTGL antibody resulted in suppression of LPL-induced reduction in infectivity of HCV-bearing medium, while knockdown of HTGL by siRNA led to increased HCV infectivity irrespective of LPL. HCV in medium from HTGL knockdown cells was found in fractions with a lower density. These results indicate that changes in the nature of HCV-associated lipoproteins by LPL and/or HTGL affect HCV infectivity, suggesting that association of HCV with specific lipoproteins is important for HCV infectivity.
研究了脂蛋白脂肪酶（LPL）和肝甘油三酯脂肪酶（HTGL）的脂肪分解作用对丙型肝炎病毒（HCV）感染的影响。首先，用 LPL 处理携带 HCV 基因组复制的 HuH7.5 细胞的培养基。LPL 处理导致 HCV 感染性降低，将 HCV 转移到更高的密度，并降低载脂蛋白 E 相关 HCV 的量。接下来，研究了 HuH7.5 分泌的内源性 HTGL 对 HCV 感染性的影响。抗 HTGL 抗体中和 HTGL 导致 LPL 诱导的含 HCV 培养基感染性降低受到抑制，而 siRNA 敲低 HTGL 导致 HCV 感染性增加，而与 LPL 无关。在 HTGL 敲低细胞的培养基中发现 HCV 存在于密度较低的部分。这些结果表明，LPL 和/或 HTGL 改变 HCV 相关脂蛋白的性质会影响 HCV 感染性，表明 HCV 与特定脂蛋白的结合对于 HCV 感染性很重要。
