病毒诱导的糖尿病。X. 脑心肌炎病毒的附着以及培养的胰腺β细胞对感染的易感性。
Virus-induced diabetes mellitus. X. Attachment of encephalomyocarditis virus and permissiveness of cultured pancreatic beta cells to infection.
作者信息
Chairez R, Yoon J W, Notkins A L
出版信息
Virology. 1978 Apr;85(2):606-11. doi: 10.1016/0042-6822(78)90465-8.
Monolayers of pancreatic β-cells from strains of mice susceptible (SJL/J) and resistant (C57BL/6J) to the development of virus-induced diabetes mellitus were inoculated with the M variant of encephalomyocarditis (EMC) virus. Immunofluorescence showed that viral antigens appeared in up to 10 times more β cells from susceptible SJL/J mice than from resistant C57BL/6J mice. Infectious center assays revealed that 10–30 times more SJL/J β cells contained infectious virus than C57BL/6J β cells. Viral attachment experiments showed no difference in the binding of EMC virus when embryonic fibroblasts, pancreatic fibroblasts, and kidney cells from SJL/J and C57BL/6J mice were compared. However, at least twice as much virus attached to the pancreatic β cells from susceptible than from resistant strains of mice. Our data suggest that genetically determined differences in viral receptors on the surface of β cells may be one of the factors controlling susceptibility to EMC-induced diabetes mellitus.
用脑心肌炎（EMC）病毒的M变种接种对病毒诱导的糖尿病敏感（SJL/J）和抗性（C57BL/6J）的小鼠品系的胰腺β细胞单层。免疫荧光显示，与抗性C57BL/6J小鼠相比，易感SJL/J小鼠的β细胞中出现病毒抗原的数量多达10倍。感染中心测定显示，含有感染性病毒的SJL/Jβ细胞比C57BL/6Jβ细胞多10 - 30倍。病毒附着实验表明，当比较SJL/J和C57BL/6J小鼠的胚胎成纤维细胞、胰腺成纤维细胞和肾细胞时，EMC病毒的结合没有差异。然而，附着在易感小鼠品系胰腺β细胞上的病毒至少是抗性品系的两倍。我们的数据表明，β细胞表面病毒受体的遗传决定差异可能是控制对EMC诱导的糖尿病易感性的因素之一。
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