重组水蛭素比活性的测定。

Determination of the specific activity of recombinant hirudin.

作者信息

Eric D

机构信息

Department of Molecular Biology, Transgene S.A., Strasbourg, France.

出版信息

Thromb Res. 1990 Dec 15;60(6):433-43. doi: 10.1016/0049-3848(90)90228-5.

DOI:10.1016/0049-3848(90)90228-5

PMID:2082477

Abstract

The anticipated importance of recombinant hirudin as an anti-thrombotic agent necessitates the development of tools for the quantification of its biological activity. A reproducible method for the determination of the specific activity of hirudin using a sensitive chromogenic assay is described. Purified recombinant hirudin proved to be close to 100% active versus thrombin. The method can also be used to determine the thrombin concentration if the hirudin concentration is known.

摘要

重组水蛭素作为一种抗血栓药物的预期重要性使得开发用于定量其生物活性的工具成为必要。本文描述了一种使用灵敏的显色测定法来测定水蛭素比活性的可重现方法。经证明，纯化的重组水蛭素对凝血酶的活性接近100%。如果已知水蛭素浓度，该方法也可用于测定凝血酶浓度。