Morishige T, Yoshioka Y, Inakura H, Tanabe A, Yao X, Tsunoda S, Tsutsumi Y, Mukai Y, Okada N, Nakagawa S
Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
Pharmazie. 2010 Aug;65(8):596-9.
Recent studies have indicated that amorphous silica particles (SPs) show cytotoxicity against various types of cells, including macrophages. However, the mechanism of cell death has not been determined, and systematic investigations of the relationship between particle characteristics and cytotoxicity are still quite limited. Here, we compared the cytotoxicity of SPs of various sizes (30-1000 nm) and surface properties against differentiated THP-1 human macrophage-like cells. We found that 300 and 1000 nm SPs showed cytotoxicity against THP-1 cells, whereas 30, 50, and 70 nm SPs did not induce cell death. We demonstrated that 1000 nm SP showed strong cytotoxicity that depended on reactive oxygen species but was independent of caspases. Furthermore, we showed that surface modification of 1000 nm SPs dramatically suppressed their cytotoxicity. Our results suggest that systematic evaluation of the association between particle characteristics and biological effects is necessary for the creation of safe SPs.
最近的研究表明，无定形二氧化硅颗粒（SPs）对包括巨噬细胞在内的各种类型细胞具有细胞毒性。然而，细胞死亡的机制尚未确定，并且关于颗粒特性与细胞毒性之间关系的系统研究仍然相当有限。在此，我们比较了各种尺寸（30 - 1000 nm）和表面性质的SPs对分化的THP - 1人巨噬细胞样细胞的细胞毒性。我们发现300和1000 nm的SPs对THP - 1细胞具有细胞毒性，而30、50和70 nm的SPs未诱导细胞死亡。我们证明1000 nm的SPs表现出强烈的细胞毒性，其依赖于活性氧但不依赖于半胱天冬酶。此外，我们表明对1000 nm的SPs进行表面修饰可显著抑制其细胞毒性。我们的结果表明，对于创建安全的SPs，有必要对颗粒特性与生物学效应之间的关联进行系统评估。
