Laboratory of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
Biochem Biophys Res Commun. 2010 Feb 5;392(2):160-5. doi: 10.1016/j.bbrc.2009.12.178. Epub 2010 Jan 7.
Although titanium dioxide (TiO2) is widely used, its inhalation can induce inflammatory diseases accompanied by interleukin-1beta (IL-1beta) production. The particle characteristics of TiO2 are important factors in its biological effects. It is urgently necessary to investigate the relationship between the particle characteristics and biological responses for the development of safe forms of TiO2. Here, we systematically compared the production of IL-1beta in response to various forms of TiO2 by macrophage-like human THP-1 cells using various sizes (nano to micro), crystal structures (anatase or rutile), and shapes (spherical or spicular) of TiO2. The production of IL-1beta depended dramatically on the characteristics of the TiO2. Notably, smaller anatase and larger rutile particles provoked higher IL-1beta production. In addition, IL-1beta production depended on active cathepsin B and reactive oxygen species production independent of the characteristics of TiO2. Our results provide basic information for the creation of safe and effective novel forms of TiO2.
尽管二氧化钛 (TiO2) 被广泛应用，但它的吸入会引起炎症性疾病，并伴有白细胞介素-1β（IL-1β）的产生。TiO2 的颗粒特性是其生物效应的重要因素。迫切需要研究颗粒特性与生物反应之间的关系，以开发安全形式的 TiO2。在这里，我们使用不同大小（纳米到微米）、晶体结构（锐钛矿或金红石）和形状（球形或针状）的 TiO2，系统地比较了巨噬细胞样人 THP-1 细胞对各种形式 TiO2 的 IL-1β 产生情况。IL-1β 的产生明显取决于 TiO2 的特性。值得注意的是，较小的锐钛矿和较大的金红石颗粒会引起更高的 IL-1β 产生。此外，IL-1β 的产生取决于活性组织蛋白酶 B 和活性氧物质的产生，而与 TiO2 的特性无关。我们的结果为创造安全有效的新型 TiO2 形式提供了基础信息。
